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Complexin-1 regulated transition in the assembly of single neuronal SNARE complex

Tongrui Hao, Nan Feng, Fan Gong, Jiaquan Liu, Lu Ma, Yu-Xuan Ren
doi: https://doi.org/10.1101/2021.06.07.447331
Tongrui Hao
1State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China
2University of Chinese Academy of Sciences, Beijing 100049, China
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  • For correspondence: haotongrui2018@sibcb.ac.cn liujiaquan@sibcb.ac.cn luma@iphy.ac.cn yxren@fudan.edu.cn
Nan Feng
1State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China
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Fan Gong
3National Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
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Jiaquan Liu
1State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China
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  • For correspondence: haotongrui2018@sibcb.ac.cn liujiaquan@sibcb.ac.cn luma@iphy.ac.cn yxren@fudan.edu.cn
Lu Ma
4Beijing National Laboratory for Condensed Matter Physics, and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China
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  • For correspondence: haotongrui2018@sibcb.ac.cn liujiaquan@sibcb.ac.cn luma@iphy.ac.cn yxren@fudan.edu.cn
Yu-Xuan Ren
5Institute for Translational Brain Research, Shanghai Medical School, Fudan University, Shanghai 200031, China
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  • For correspondence: haotongrui2018@sibcb.ac.cn liujiaquan@sibcb.ac.cn luma@iphy.ac.cn yxren@fudan.edu.cn
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ABSTRACT

Neurotransmitter release is mediated by the synaptic vesicle exocytosis. Important proteins in this process have been identified including the molecular machine Synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins, and other regulators. Complexin (Cpx) is one of the vital regulators in this process. The functions of Cpx are proposed to maintain a proper primed vesicle pool by preventing its premature depletion, which facilitates the vesicle fusion in the presence of Ca2+. However, the molecular mechanism remains unclear. Using dual-trap optical tweezers, we detected the interaction of complexin-1 (CpxI) with SNARE. We found that the CpxI stabilizes partially folded SNARE complexes by competing with C-terminal of Vamp protein and interacting with the C-terminal of t-SNARE complex.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted June 07, 2021.
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Complexin-1 regulated transition in the assembly of single neuronal SNARE complex
Tongrui Hao, Nan Feng, Fan Gong, Jiaquan Liu, Lu Ma, Yu-Xuan Ren
bioRxiv 2021.06.07.447331; doi: https://doi.org/10.1101/2021.06.07.447331
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Complexin-1 regulated transition in the assembly of single neuronal SNARE complex
Tongrui Hao, Nan Feng, Fan Gong, Jiaquan Liu, Lu Ma, Yu-Xuan Ren
bioRxiv 2021.06.07.447331; doi: https://doi.org/10.1101/2021.06.07.447331

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