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NanoDam identifies novel temporal transcription factors conserved between the Drosophila central brain and visual system

View ORCID ProfileJocelyn L.Y. Tang, View ORCID ProfileAnna E. Hakes, View ORCID ProfileRobert Krautz, View ORCID ProfileTakumi Suzuki, View ORCID ProfileEsteban G. Contreras, View ORCID ProfilePaul M. Fox, View ORCID ProfileAndrea H. Brand
doi: https://doi.org/10.1101/2021.06.07.447332
Jocelyn L.Y. Tang
The Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK
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  • ORCID record for Jocelyn L.Y. Tang
Anna E. Hakes
1UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA 94158, USA
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Robert Krautz
2The Bioinformatics Centre, Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark
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Takumi Suzuki
3The College of Science, Department of Science, Ibaraki University, 1-1-2 Bunkyo, Mito, Ibaraki, 310-8512, Japan
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Esteban G. Contreras
4Institut für Neuro- und Verhaltensbiologie, Universität Münster, Badestreet 9, 48149, Münster, Germany
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Paul M. Fox
5AbbVie, Cruce Dávila, Barceloneta, 00652, Puerto Rico, US
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Andrea H. Brand
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  • For correspondence: a.brand@gurdon.cam.ac.uk
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Summary

Temporal patterning of neural progenitors is an evolutionarily conserved strategy for generating neuronal diversity. Type II neural stem cells in the Drosophila central brain produce transit-amplifying intermediate neural progenitors (INPs) that exhibit temporal patterning. However, the known temporal factors cannot account for the neuronal diversity in the adult brain. To search for new temporal factors, we developed NanoDam, which enables rapid genome-wide profiling of endogenously-tagged proteins in vivo with a single genetic cross. Mapping the targets of known temporal transcription factors with NanoDam identified Homeobrain and Scarecrow (ARX and NKX2.1 orthologues) as novel temporal factors. We show that Homeobrain and Scarecrow define middle-aged and late INP temporal windows and play a role in cellular longevity. Strikingly, Homeobrain and Scarecrow have conserved functions as temporal factors in the developing visual system. NanoDam enables rapid cell type-specific genome-wide profiling with temporal resolution and can be easily adapted for use in higher organisms.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 08, 2021.
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NanoDam identifies novel temporal transcription factors conserved between the Drosophila central brain and visual system
Jocelyn L.Y. Tang, Anna E. Hakes, Robert Krautz, Takumi Suzuki, Esteban G. Contreras, Paul M. Fox, Andrea H. Brand
bioRxiv 2021.06.07.447332; doi: https://doi.org/10.1101/2021.06.07.447332
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NanoDam identifies novel temporal transcription factors conserved between the Drosophila central brain and visual system
Jocelyn L.Y. Tang, Anna E. Hakes, Robert Krautz, Takumi Suzuki, Esteban G. Contreras, Paul M. Fox, Andrea H. Brand
bioRxiv 2021.06.07.447332; doi: https://doi.org/10.1101/2021.06.07.447332

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