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Single-molecule imaging reveals the coupling of translation and mRNA decay

Pratik Dave, Esther Griesbach, Gregory Roth, Daniel Mateju, View ORCID ProfileJeffrey A. Chao
doi: https://doi.org/10.1101/2021.06.07.447377
Pratik Dave
1Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
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Esther Griesbach
1Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
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Gregory Roth
1Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
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Daniel Mateju
1Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
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Jeffrey A. Chao
1Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
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  • ORCID record for Jeffrey A. Chao
  • For correspondence: jeffrey.chao@fmi.ch
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SUMMARY

The relationship between mRNA translation and decay is incompletely understood, with conflicting reports suggesting that translation can either promote decay or stabilize mRNAs. The effect of translation on mRNA decay has mainly been studied using ensemble measurements and global inhibitors of transcription and translation, which can mask the underlying mechanisms. We developed a single-molecule imaging approach to control the translation of a specific transcript that enabled simultaneous measurement of translation and mRNA decay. Our results demonstrate that mRNAs undergoing translation are degraded faster than non-translating ones, although with slower kinetics than translation-coupled degradation of transcripts targeted by NMD. Furthermore, our results indicate that miRNAs mediate efficient degradation of both translating and non-translating target mRNAs. Single-molecule measurements of translation and decay reveal a predominant role of mRNA decay in miRNA-mediated regulation. Simultaneous visualization of translation and decay on single mRNAs provides a framework to study how these processes are interconnected in cells.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted June 07, 2021.
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Single-molecule imaging reveals the coupling of translation and mRNA decay
Pratik Dave, Esther Griesbach, Gregory Roth, Daniel Mateju, Jeffrey A. Chao
bioRxiv 2021.06.07.447377; doi: https://doi.org/10.1101/2021.06.07.447377
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Single-molecule imaging reveals the coupling of translation and mRNA decay
Pratik Dave, Esther Griesbach, Gregory Roth, Daniel Mateju, Jeffrey A. Chao
bioRxiv 2021.06.07.447377; doi: https://doi.org/10.1101/2021.06.07.447377

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