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The ChAdOx1 vectored vaccine, AZD2816, induces strong immunogenicity against SARS-CoV-2 Beta (B.1.351) and other variants of concern in preclinical studies

View ORCID ProfileAlexandra J Spencer, Susan Morris, View ORCID ProfileMarta Ulaszewska, Claire Powers, Reshma Kailath, View ORCID ProfileCameron Bissett, Adam Truby, View ORCID ProfileNazia Thakur, View ORCID ProfileJoseph Newman, View ORCID ProfileElizabeth R Allen, View ORCID ProfileIndra Rudiansyah, Chang Liu, Wanwisa Dejnirattisai, Juthathip Mongkolsapaya, Hannah Davies, View ORCID ProfileFrancesca R Donnellan, David Pulido, View ORCID ProfileThomas P. Peacock, View ORCID ProfileWendy S. Barclay, Helen Bright, Kuishu Ren, Gavin Screaton, Patrick McTamney, View ORCID ProfileDalan Bailey, View ORCID ProfileSarah C Gilbert, View ORCID ProfileTeresa Lambe
doi: https://doi.org/10.1101/2021.06.08.447308
Alexandra J Spencer
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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  • For correspondence: alex.spencer@ndm.ox.ac.uk
Susan Morris
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Marta Ulaszewska
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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  • ORCID record for Marta Ulaszewska
Claire Powers
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Reshma Kailath
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Cameron Bissett
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Adam Truby
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Nazia Thakur
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
2The Pirbright Institute, Woking, Surrey, United Kingdom
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Joseph Newman
2The Pirbright Institute, Woking, Surrey, United Kingdom
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Elizabeth R Allen
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Indra Rudiansyah
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Chang Liu
3The Wellcome Trust Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, United Kingdom
4Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, United Kingdom
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Wanwisa Dejnirattisai
3The Wellcome Trust Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Juthathip Mongkolsapaya
3The Wellcome Trust Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Hannah Davies
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Francesca R Donnellan
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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David Pulido
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Thomas P. Peacock
5Department of Infectious Disease, Imperial College London, United Kingdom
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Wendy S. Barclay
5Department of Infectious Disease, Imperial College London, United Kingdom
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Helen Bright
6Virology and Vaccine Discovery, Microbial Sciences, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD United States
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Kuishu Ren
6Virology and Vaccine Discovery, Microbial Sciences, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD United States
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Gavin Screaton
3The Wellcome Trust Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Patrick McTamney
6Virology and Vaccine Discovery, Microbial Sciences, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD United States
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Dalan Bailey
2The Pirbright Institute, Woking, Surrey, United Kingdom
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  • ORCID record for Dalan Bailey
Sarah C Gilbert
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
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Teresa Lambe
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
4Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, United Kingdom
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Abstract

There is an ongoing global effort to design, manufacture, and clinically assess vaccines against SARS-CoV-2. Over the course of the ongoing pandemic a number of new SARS-CoV-2 virus isolates or variants of concern (VoC) have been identified containing mutations in key proteins. In this study we describe the generation and preclinical assessment of a ChAdOx1-vectored vaccine (AZD2816) which expresses the spike protein of the Beta VoC (B.1.351). We demonstrate that AZD2816 is immunogenic after a single dose. When AZD2816 is used as a booster dose in animals primed with a vaccine encoding the original spike protein (ChAdOx1 nCoV-19/ [AZD1222]), high titre binding and neutralising antibodies against Beta (B.1.351), Gamma (P.1) and Delta (B.1.617.2) are induced. In addition, a strong and polyfunctional T cell response was measured in these booster regimens. These data support the ongoing clinical development and testing of this new variant vaccine.

Competing Interest Statement

SCG is co-founder and board member of Vaccitech and named as an inventor on a patent covering use of ChAdOx1-vectored vaccines and a patent application covering the ChAdOx1 nCoV-19 (AZD1222) vaccine. TL is named as an inventor on a patent application covering the ChAdOx1 nCoV-19 (AZD1222) vaccine and was consultant to Vaccitech. PM was an employee of AstraZeneca, KR is an employee of AstraZeneca. HB is an employee of AstraZeneca and is a named inventor on a patent application covering the AZD2816 vaccine.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted September 09, 2021.
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The ChAdOx1 vectored vaccine, AZD2816, induces strong immunogenicity against SARS-CoV-2 Beta (B.1.351) and other variants of concern in preclinical studies
Alexandra J Spencer, Susan Morris, Marta Ulaszewska, Claire Powers, Reshma Kailath, Cameron Bissett, Adam Truby, Nazia Thakur, Joseph Newman, Elizabeth R Allen, Indra Rudiansyah, Chang Liu, Wanwisa Dejnirattisai, Juthathip Mongkolsapaya, Hannah Davies, Francesca R Donnellan, David Pulido, Thomas P. Peacock, Wendy S. Barclay, Helen Bright, Kuishu Ren, Gavin Screaton, Patrick McTamney, Dalan Bailey, Sarah C Gilbert, Teresa Lambe
bioRxiv 2021.06.08.447308; doi: https://doi.org/10.1101/2021.06.08.447308
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The ChAdOx1 vectored vaccine, AZD2816, induces strong immunogenicity against SARS-CoV-2 Beta (B.1.351) and other variants of concern in preclinical studies
Alexandra J Spencer, Susan Morris, Marta Ulaszewska, Claire Powers, Reshma Kailath, Cameron Bissett, Adam Truby, Nazia Thakur, Joseph Newman, Elizabeth R Allen, Indra Rudiansyah, Chang Liu, Wanwisa Dejnirattisai, Juthathip Mongkolsapaya, Hannah Davies, Francesca R Donnellan, David Pulido, Thomas P. Peacock, Wendy S. Barclay, Helen Bright, Kuishu Ren, Gavin Screaton, Patrick McTamney, Dalan Bailey, Sarah C Gilbert, Teresa Lambe
bioRxiv 2021.06.08.447308; doi: https://doi.org/10.1101/2021.06.08.447308

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