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Populus ERF85 balances xylem cell expansion and secondary cell wall formation in hybrid aspen

View ORCID ProfileCarolin Seyfferth, View ORCID ProfileBernard A. Wessels, Jorma Vahala, View ORCID ProfileJaakko Kangasjärvi, View ORCID ProfileNicolas Delhomme, View ORCID ProfileTorgeir R. Hvidsten, View ORCID ProfileHannele Tuominen, View ORCID ProfileJudith Lundberg-Felten
doi: https://doi.org/10.1101/2021.06.08.447517
Carolin Seyfferth
1Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187 Umeå, Sweden
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  • ORCID record for Carolin Seyfferth
Bernard A. Wessels
1Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187 Umeå, Sweden
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Jorma Vahala
3Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, FI-00014 Helsinki, Finland
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Jaakko Kangasjärvi
3Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, FI-00014 Helsinki, Finland
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Nicolas Delhomme
2Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90184 Umeå, Sweden
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Torgeir R. Hvidsten
1Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187 Umeå, Sweden
4Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway
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Hannele Tuominen
2Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90184 Umeå, Sweden
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Judith Lundberg-Felten
2Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90184 Umeå, Sweden
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  • For correspondence: judith.lundberg-felten@slu.se
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Abstract

Secondary growth relies on precise and specialized transcriptional networks that determine cell division, differentiation, and maturation of xylem cells. We identify a novel role for the ethylene induced Populus ETHYLENE RESPONSE FACTOR ERF85 (Potri.015G023200) in balancing xylem cell expansion and secondary cell wall (SCW) formation in hybrid aspen (Populus tremula x tremuloides). Expression of ERF85 is high in phloem and cambium cells and during expansion of xylem cells, while it is low in maturing xylem tissue. Extending ERF85 expression into SCW forming zones of woody tissues through ectopic expression reduced wood density and SCW thickness of xylem fibers but increased fiber diameter. Xylem transcriptomes from the transgenic trees revealed transcriptional induction of genes involved in cell expansion, translation and growth. Expression of genes associated with plant vascular development and biosynthesis of SCW chemical components such as xylan and lignin, was downregulated in the transgenic trees. Our results suggest that ERF85 activates genes related with xylem cell expansion, while preventing transcriptional activation of genes related to SCW formation. The importance of precise spatial expression of ERF85 during wood development together with the observed phenotypes in response to ectopic ERF85 expression suggests that ERF85 functions as a switch between different phases of xylem differentiation during wood formation.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://github.com/carSeyff/ERF85OE

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 09, 2021.
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Populus ERF85 balances xylem cell expansion and secondary cell wall formation in hybrid aspen
Carolin Seyfferth, Bernard A. Wessels, Jorma Vahala, Jaakko Kangasjärvi, Nicolas Delhomme, Torgeir R. Hvidsten, Hannele Tuominen, Judith Lundberg-Felten
bioRxiv 2021.06.08.447517; doi: https://doi.org/10.1101/2021.06.08.447517
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Populus ERF85 balances xylem cell expansion and secondary cell wall formation in hybrid aspen
Carolin Seyfferth, Bernard A. Wessels, Jorma Vahala, Jaakko Kangasjärvi, Nicolas Delhomme, Torgeir R. Hvidsten, Hannele Tuominen, Judith Lundberg-Felten
bioRxiv 2021.06.08.447517; doi: https://doi.org/10.1101/2021.06.08.447517

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