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High resolution dynamic mapping of the C. elegans intestinal brush border

View ORCID ProfileAurélien Bidaud-Meynard, Flora Demouchy, Ophélie Nicolle, Anne Pacquelet, Shashi Kumar Suman, Camille Plancke, View ORCID ProfileFrançois Robin, View ORCID ProfileGrégoire Michaux
doi: https://doi.org/10.1101/2021.06.14.448317
Aurélien Bidaud-Meynard
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) - UMR 6290, F-35000 Rennes, France
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  • ORCID record for Aurélien Bidaud-Meynard
Flora Demouchy
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) - UMR 6290, F-35000 Rennes, France
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Ophélie Nicolle
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) - UMR 6290, F-35000 Rennes, France
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Anne Pacquelet
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) - UMR 6290, F-35000 Rennes, France
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Shashi Kumar Suman
2Sorbonne Université, Inserm, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire de Biologie du développement, F-75005, Paris, France
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Camille Plancke
2Sorbonne Université, Inserm, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire de Biologie du développement, F-75005, Paris, France
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François Robin
2Sorbonne Université, Inserm, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire de Biologie du développement, F-75005, Paris, France
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Grégoire Michaux
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes) - UMR 6290, F-35000 Rennes, France
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  • For correspondence: gmichaux@univ-rennes1.fr
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Abstract

The intestinal brush border is made of an array of microvilli that increases the membrane surface area for nutrient processing, absorption, and host defence. Studies on mammalian cultured epithelial cells uncovered some of the molecular players and physical constrains required to establish this apical specialized membrane. However, the building and maintenance of a brush border in vivo has not been investigated in detail yet. Here, we combined super-resolution imaging, transmission electron microscopy and genome editing in the developing nematode C. elegans to build a high-resolution and dynamic localization map of known and new markers of the brush border. Notably, we show that microvilli components are dynamically enriched at the apical membrane during microvilli outgrowth and maturation but become highly stable once microvilli are built. This new mapping tool will be instrumental to understand the molecular processes of microvilli growth and maintenance in vivo as well as the effect of genetic perturbations, notably in the context of disorders affecting brush border integrity.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵* lead contact gmichaux{at}univ-rennes1.fr

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 20, 2021.
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High resolution dynamic mapping of the C. elegans intestinal brush border
Aurélien Bidaud-Meynard, Flora Demouchy, Ophélie Nicolle, Anne Pacquelet, Shashi Kumar Suman, Camille Plancke, François Robin, Grégoire Michaux
bioRxiv 2021.06.14.448317; doi: https://doi.org/10.1101/2021.06.14.448317
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High resolution dynamic mapping of the C. elegans intestinal brush border
Aurélien Bidaud-Meynard, Flora Demouchy, Ophélie Nicolle, Anne Pacquelet, Shashi Kumar Suman, Camille Plancke, François Robin, Grégoire Michaux
bioRxiv 2021.06.14.448317; doi: https://doi.org/10.1101/2021.06.14.448317

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