Abstract
Mycobacterium avium subsp. hominissuis (MAH) is one of the most important agents causing non-tuberculosis mycobacterial infection in humans and pigs. Genome analysis on MAH of human isolates has been proceeding, however, those of pigs are limited despite its potential source of infection to human. In the current study, we obtained 30 draft genome sequences of MAH of pigs reared in Japan. The 30 draft genomes consisted of 4,848,678 – 5,620,788 bp length, 4,652 – 5,388 coding genes and 46 – 75 (Med: 47) tRNAs. All isolates had restriction modification associated genes and 185 – 222 predicted virulence genes. Two isolates had tRNA arrays and one isolate had a clustered regularly interspaced short palindromic repeat (CRISPR) region. Our results will be useful for evaluation of the ecology of MAH by providing a foundation for genome-based epidemiological studies.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
E-mail addresses and ORCID IDs: Tetsuya Komatsu: gsbqm670{at}yahoo.co.jp
Kenji Ohya: kenji.ohya{at}kkd.biglobe.ne.jp
Atsushi Ota: level997{at}gmail.com
Yukiko Nishiuchi: nishiuchi{at}hiroshima-u.ac.jp
Hirokazu Yano: yano.hirokazu{at}ige.tohoku.ac.jp
Kayoko Matsuo: alaeuris{at}violin.ocn.ne.jp
Justice Opare Odoi: wentworthprince{at}yahoo.com
Shota Suganuma: suganuma-shouta{at}zennoh.or.jp
Kotaro Sawai: sawaik107{at}affrc.go.jp
Akemi Hasebe: akemi.hasebe{at}pref.toyama.lg.jp
Tetsuo Asai: tasai{at}gifu-u.ac.jp
Tokuma Yanai: tokumayanai{at}gmail.com
Hideto Fukushi: hfukushi{at}gifu-u.ac.jp
Takayuki Wada: twada{at}osaka-cu.ac.jp
Shiomi Yoshida: yoshida.shiomi.vg{at}mail.hosp.go.jp
Toshihiro Ito: toshihiroito{at}nibiohn.go.jp
Kentaro Arikawa: kentaro_arikawa{at}office.city.kobe.lg.jp
Mikihiko Kawai: kawai.mikihiko.8c{at}kyoto-u.ac.jp
Manabu Ato: ato{at}niid.go.jp
Anthony D Baughn: abaughn{at}umn.edu
Tomotada Iwamoto: kx2t-iwmt{at}asahi-net.or.jp
Fumito Maruyama: fumito{at}hiroshima-u.ac.jp
Research Areas: Microbiology
Method g) revised; Figure 1 revised; Supplementary Table 2 deleted, Supplementary Table 5 revised.
List of abbreviations
- NTM
- non-tuberculous mycobacteria
- MAC
- Mycobacterium avium complex
- MAA
- M. avium subsp. avium
- MAP
- M. avium subsp. paratuberculosis
- MAS
- M. avium subsp. silvaticum
- MAH
- Mycobacterium avium subsp. hominissuis
- MTB
- Mycobacterium tuberculosis
- RM
- restriction modification
- CRISPR
- clustered regularly interspaced short palindromic repeat
- BLAST
- Basic Local Alignment Search Tool
- MEGA
- Molecular Evolutionary Genetics Analysis
- NCBI
- National Center for Biotechnology Information
- PGAP
- Prokaryotic Genome Annotation Pipeline
- SNP
- single nucleotide polymorphism
