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SARS-CoV-2 Viral Replication in a High Throughput Human Primary Epithelial Airway Organ Model

Christine R. Fisher, Felix Mba Medie, Rebeccah J. Luu, Robert Gaibler, Caitlin R. Miller, Thomas J. Mulhern, Vidhya Vijayakumar, Elizabeth Marr, Jehan Alladina, Benjamin Medoff, View ORCID ProfileJeffrey T. Borenstein, Ashley L. Gard
doi: https://doi.org/10.1101/2021.06.15.448611
Christine R. Fisher
1Bioengineering Division, Draper, Cambridge MA 02139
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Felix Mba Medie
1Bioengineering Division, Draper, Cambridge MA 02139
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Rebeccah J. Luu
1Bioengineering Division, Draper, Cambridge MA 02139
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Robert Gaibler
1Bioengineering Division, Draper, Cambridge MA 02139
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Caitlin R. Miller
1Bioengineering Division, Draper, Cambridge MA 02139
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Thomas J. Mulhern
1Bioengineering Division, Draper, Cambridge MA 02139
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Vidhya Vijayakumar
1Bioengineering Division, Draper, Cambridge MA 02139
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Elizabeth Marr
1Bioengineering Division, Draper, Cambridge MA 02139
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Jehan Alladina
2Department of Pulmonary Critical Care Medicine, Massachusetts General Hospital, Boston MA
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Benjamin Medoff
2Department of Pulmonary Critical Care Medicine, Massachusetts General Hospital, Boston MA
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Jeffrey T. Borenstein
1Bioengineering Division, Draper, Cambridge MA 02139
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  • ORCID record for Jeffrey T. Borenstein
Ashley L. Gard
1Bioengineering Division, Draper, Cambridge MA 02139
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  • For correspondence: agard@draper.com
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Abstract

COVID-19 emerged as a worldwide pandemic early in 2020, and at this writing has caused over 170 million cases and 3.7 million deaths worldwide, and almost 600,000 deaths in the United States. The rapid development of several safe and highly efficacious vaccines stands as one of the most extraordinary achievements in modern medicine, but the identification and administration of efficacious therapeutics to treat patients suffering from COVID-19 has been far less successful. A major factor limiting progress in the development of effective treatments has been a lack of suitable preclinical models for the disease, currently reliant upon various animal models and in vitro culture of immortalized cell lines. Here we report the first successful demonstration of SARS-CoV-2 infection and viral replication in a human primary cell-based organ-on-chip, leveraging a recently developed tissue culture platform known as PREDICT96. This successful demonstration of SARS-CoV-2 infection in human primary airway epithelial cells derived from a living donor represents a powerful new pathway for disease modeling and an avenue for screening therapeutic candidates in a high throughput platform.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted June 16, 2021.
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SARS-CoV-2 Viral Replication in a High Throughput Human Primary Epithelial Airway Organ Model
Christine R. Fisher, Felix Mba Medie, Rebeccah J. Luu, Robert Gaibler, Caitlin R. Miller, Thomas J. Mulhern, Vidhya Vijayakumar, Elizabeth Marr, Jehan Alladina, Benjamin Medoff, Jeffrey T. Borenstein, Ashley L. Gard
bioRxiv 2021.06.15.448611; doi: https://doi.org/10.1101/2021.06.15.448611
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SARS-CoV-2 Viral Replication in a High Throughput Human Primary Epithelial Airway Organ Model
Christine R. Fisher, Felix Mba Medie, Rebeccah J. Luu, Robert Gaibler, Caitlin R. Miller, Thomas J. Mulhern, Vidhya Vijayakumar, Elizabeth Marr, Jehan Alladina, Benjamin Medoff, Jeffrey T. Borenstein, Ashley L. Gard
bioRxiv 2021.06.15.448611; doi: https://doi.org/10.1101/2021.06.15.448611

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