Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Measles fusion complexes from central nervous system clinical isolates: decreased interaction between hemagglutinin and fusion proteins

View ORCID ProfileCyrille Mathieu, Tiago Nascimento Figueira, Amanda R. Decker, Marion Ferren, Tiziana F. Bovier, Eric M. Jurgens, Tara C. Marcink, Anne Moscona, View ORCID ProfileMatteo Porotto
doi: https://doi.org/10.1101/2021.06.18.449082
Cyrille Mathieu
1Center for Host-Pathogen Interaction, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
2Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
3CIRI, Centre International de Recherche en Infectiologie, Team Immunobiology of the Viral infections, Univ Lyon, Inserm, U1111, CNRS, UMR5308, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, 69007, Lyon, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Cyrille Mathieu
  • For correspondence: cyrille.mathieu@inserm.fr mp3509@cumc.columbia.edu
Tiago Nascimento Figueira
1Center for Host-Pathogen Interaction, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
2Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amanda R. Decker
1Center for Host-Pathogen Interaction, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
2Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marion Ferren
1Center for Host-Pathogen Interaction, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
2Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
3CIRI, Centre International de Recherche en Infectiologie, Team Immunobiology of the Viral infections, Univ Lyon, Inserm, U1111, CNRS, UMR5308, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, 69007, Lyon, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tiziana F. Bovier
1Center for Host-Pathogen Interaction, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
2Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
4Department of Experimental Medicine, University of Campania, 81100 Caserta, Caserta, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric M. Jurgens
1Center for Host-Pathogen Interaction, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
2Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
5Weill Cornell Medical College, New York, New York, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tara C. Marcink
1Center for Host-Pathogen Interaction, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
2Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anne Moscona
1Center for Host-Pathogen Interaction, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
2Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
6Department of Microbiology & Immunology, Columbia University Vagelos College of Physicians and Surgeons, New York, New York, USA
7Department of Physiology & Cellular Biophysics, Columbia University Vagelos College of Physicians and Surgeons, New York, New York, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matteo Porotto
1Center for Host-Pathogen Interaction, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
2Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons, New York, New York USA
4Department of Experimental Medicine, University of Campania, 81100 Caserta, Caserta, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Matteo Porotto
  • For correspondence: cyrille.mathieu@inserm.fr mp3509@cumc.columbia.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Measles virus (MeV) viral entry is mediated by a fusion complex comprised of a receptor binding protein (hemagglutinin, H) and a fusion protein (F). The wild type H/F complex requires interaction with specific proteinaceous receptors (CD150/SLAM and nectin-4) in order to be activated. In contrast the H/F complexes isolated from viruses infecting the central nervous system (CNS) do not require a specific receptor. A single amino acid change in the F protein (L454W) was previously identified in two patients with lethal sequelae of MeV CNS infection, and the F bearing this mutation mediates fusion even without the H protein. We show here that viruses bearing the L454W fusion complex are less efficient than wt virus at targeting receptor expressing cells and that this defect is associated with a decreased interaction between the H and the F proteins.

Importance Measles (Mev) infection can cause serious complications including measles inclusion body encephalitis (MIBE) and subacute sclerosing panencephalitis (SSPE). MIBE and SSPE are relatively rare but lethal. We have shown that the fusion complex of CNS adapted clinical samples can spread in the absence of known receptor. We now provide evidence that HRC mutations leading to CNS adaptation come at a cost to the efficiency of viral entry.

One Sentence Summary Measles CNS adapted fusion complexes have altered H/F interaction.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted June 19, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Measles fusion complexes from central nervous system clinical isolates: decreased interaction between hemagglutinin and fusion proteins
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Measles fusion complexes from central nervous system clinical isolates: decreased interaction between hemagglutinin and fusion proteins
Cyrille Mathieu, Tiago Nascimento Figueira, Amanda R. Decker, Marion Ferren, Tiziana F. Bovier, Eric M. Jurgens, Tara C. Marcink, Anne Moscona, Matteo Porotto
bioRxiv 2021.06.18.449082; doi: https://doi.org/10.1101/2021.06.18.449082
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Measles fusion complexes from central nervous system clinical isolates: decreased interaction between hemagglutinin and fusion proteins
Cyrille Mathieu, Tiago Nascimento Figueira, Amanda R. Decker, Marion Ferren, Tiziana F. Bovier, Eric M. Jurgens, Tara C. Marcink, Anne Moscona, Matteo Porotto
bioRxiv 2021.06.18.449082; doi: https://doi.org/10.1101/2021.06.18.449082

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3513)
  • Biochemistry (7358)
  • Bioengineering (5335)
  • Bioinformatics (20295)
  • Biophysics (10032)
  • Cancer Biology (7753)
  • Cell Biology (11326)
  • Clinical Trials (138)
  • Developmental Biology (6442)
  • Ecology (9964)
  • Epidemiology (2065)
  • Evolutionary Biology (13342)
  • Genetics (9364)
  • Genomics (12595)
  • Immunology (7717)
  • Microbiology (19057)
  • Molecular Biology (7452)
  • Neuroscience (41087)
  • Paleontology (300)
  • Pathology (1233)
  • Pharmacology and Toxicology (2140)
  • Physiology (3170)
  • Plant Biology (6867)
  • Scientific Communication and Education (1275)
  • Synthetic Biology (1899)
  • Systems Biology (5320)
  • Zoology (1089)