Abstract
The transmission of airborne pathogens via aerosols is considered to be the main route through which a number of known and emerging respiratory diseases infect their hosts. It is therefore essential to quantify airborne transmission in closed spaces and determine what recommendations should be implemented to minimize the exposure to the pathogen in built environments. We have developed a method to detect viable virus particles from aerosols by using an aerosolized bacteriophage Phi6 in combination with its host Pseudomonas phaseolicola, which when seeded on agar plates acts as a virus detector that can be placed at a range of distances away from the aerosol-generating source. Based on this method we present two striking results: (1) We consistently detected viable phage particles at distances of 18 feet away from the source within 15-minutes of exposure in a classroom equipped with a state of the art HVAC system. (2) Increasing the relative humidity beyond 40% at a maintained temperature of (22.8 ± 0.2) °C significantly reduces the risk of transmission. Our method can be used to quantify the exposure to pathogens at various distances from the source for different amounts of time, data which can be used to set safety standards for room capacity and the efficacy of interventions which aim to reduce pathogen levels in closed spaces of specified size and intended use.
Summary We present a method to experimentally determine the exposure to airborne pathogens in closed spaces.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
↵* co-first authors
