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Efficient tagging and purification of endogenous proteins for structural studies by single particle cryo-EM

View ORCID ProfileJianhua Zhao, View ORCID ProfileSuraj Makhija, View ORCID ProfileBo Huang, View ORCID ProfileYifan Cheng
doi: https://doi.org/10.1101/2021.06.25.449985
Jianhua Zhao
1Department of Biochemistry and Biophysics, University of California San Francisco. San Francisco, CA 94143, USA
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Suraj Makhija
2UC Berkeley – UCSF Joint Graduate Program in Bioengineering, University of California, San Francisco, CA 94143, USA
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Bo Huang
3Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94143, USA
4Chan Zuckerberg Biohub, San Francisco, CA 94158, USA
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  • For correspondence: Bo.Huang@ucsf.edu Yifan.Cheng@ucsf.edu
Yifan Cheng
1Department of Biochemistry and Biophysics, University of California San Francisco. San Francisco, CA 94143, USA
5Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA, 94158
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  • For correspondence: Bo.Huang@ucsf.edu Yifan.Cheng@ucsf.edu
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Article Information

doi 
https://doi.org/10.1101/2021.06.25.449985
History 
  • July 2, 2021.

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  • Version 1 (June 25, 2021 - 22:13).
  • You are viewing Version 2, the most recent version of this article.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. Jianhua Zhao1,#,*,
  2. Suraj Makhija2,&,*,
  3. Bo Huang3,4,$ and
  4. Yifan Cheng1,5,$
  1. 1Department of Biochemistry and Biophysics, University of California San Francisco. San Francisco, CA 94143, USA
  2. 2UC Berkeley – UCSF Joint Graduate Program in Bioengineering, University of California, San Francisco, CA 94143, USA
  3. 3Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94143, USA
  4. 4Chan Zuckerberg Biohub, San Francisco, CA 94158, USA
  5. 5Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA, 94158
  1. ↵$Correspondence: Bo.Huang{at}ucsf.edu and Yifan.Cheng{at}ucsf.edu
  1. ↵* These authors contributed equally

  • ↵# Cell and Molecular Biology Program, Sanford Burnham Prebys Medical Discovery Institute, San Diego, CA 92037

  • ↵& Scribe Therapeutics, Alameda, CA 94501

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Posted July 02, 2021.
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Efficient tagging and purification of endogenous proteins for structural studies by single particle cryo-EM
Jianhua Zhao, Suraj Makhija, Bo Huang, Yifan Cheng
bioRxiv 2021.06.25.449985; doi: https://doi.org/10.1101/2021.06.25.449985
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Efficient tagging and purification of endogenous proteins for structural studies by single particle cryo-EM
Jianhua Zhao, Suraj Makhija, Bo Huang, Yifan Cheng
bioRxiv 2021.06.25.449985; doi: https://doi.org/10.1101/2021.06.25.449985

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