Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

TLR4 regulation in human fetal membranes as an explicative mechanism of a pathological preterm case

Corinne Belville, Flora Ponelle-Chachuat, Marion Rouzaire, Christelle Gross, Bruno Pereira, Denis Gallot, Vincent Sapin, Loïc Blanchon
doi: https://doi.org/10.1101/2021.06.28.450131
Corinne Belville
1Team “Translational approach to epithelial injury and repair,” GReD, Université Clermont Auvergne, UMR6293 CNRS-U1103 INSERM, F-63000 Clermont-Ferrand, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Flora Ponelle-Chachuat
1Team “Translational approach to epithelial injury and repair,” GReD, Université Clermont Auvergne, UMR6293 CNRS-U1103 INSERM, F-63000 Clermont-Ferrand, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marion Rouzaire
1Team “Translational approach to epithelial injury and repair,” GReD, Université Clermont Auvergne, UMR6293 CNRS-U1103 INSERM, F-63000 Clermont-Ferrand, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christelle Gross
1Team “Translational approach to epithelial injury and repair,” GReD, Université Clermont Auvergne, UMR6293 CNRS-U1103 INSERM, F-63000 Clermont-Ferrand, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bruno Pereira
2CHU Clermont-Ferrand, Biostatistics unit (DRCI) Department, F-63000 Clermont-Ferrand, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Denis Gallot
1Team “Translational approach to epithelial injury and repair,” GReD, Université Clermont Auvergne, UMR6293 CNRS-U1103 INSERM, F-63000 Clermont-Ferrand, France
3CHU Clermont-Ferrand, Obstetrics and Gynecology Department, F-63000 Clermont-Ferrand, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vincent Sapin
1Team “Translational approach to epithelial injury and repair,” GReD, Université Clermont Auvergne, UMR6293 CNRS-U1103 INSERM, F-63000 Clermont-Ferrand, France
4CHU Clermont-Ferrand, Biochemistry and Molecular Genetic Department, F-63000 Clermont-Ferrand, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Loïc Blanchon
1Team “Translational approach to epithelial injury and repair,” GReD, Université Clermont Auvergne, UMR6293 CNRS-U1103 INSERM, F-63000 Clermont-Ferrand, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: loic.blanchon@uca.fr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

The integrity of human fetal membranes is crucial for harmonious fetal development throughout pregnancy. Their premature rupture is often the consequence of a physiological phenomenon previously exacerbated. Beyond all biological processes implied, inflammation is of primary importance and is qualified as “sterile” at the end of pregnancy. Complementary methylomic and transcriptomic strategies on amnion and choriodecidua explants taken from the altered (cervix zone) and intact fetal membranes at term and before labor were used in this study. By cross-analyzing genome-wide studies strengthened by in vitro experiments, we deciphered how the expression of Toll-like receptor 4 (TLR4), a well-known actor of pathological fetal membrane rupture, is controlled. Indeed, it is differentially regulated in the altered zone and between both layers by a dual mechanism: 1) the methylation of TLR4 and miRNA promoters and 2) targeting by miRNA (let-7a-2 and miR-125b-1) acting on the 3’-UTR of TLR4. Consequently, this study demonstrates that a fine regulation of TLR4 is required for sterile inflammation establishment at the end of pregnancy and that it may be dysregulated in the pathological premature rupture of membranes.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Figure presentations were modified. Text and figure legends were updated

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted July 05, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
TLR4 regulation in human fetal membranes as an explicative mechanism of a pathological preterm case
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
TLR4 regulation in human fetal membranes as an explicative mechanism of a pathological preterm case
Corinne Belville, Flora Ponelle-Chachuat, Marion Rouzaire, Christelle Gross, Bruno Pereira, Denis Gallot, Vincent Sapin, Loïc Blanchon
bioRxiv 2021.06.28.450131; doi: https://doi.org/10.1101/2021.06.28.450131
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
TLR4 regulation in human fetal membranes as an explicative mechanism of a pathological preterm case
Corinne Belville, Flora Ponelle-Chachuat, Marion Rouzaire, Christelle Gross, Bruno Pereira, Denis Gallot, Vincent Sapin, Loïc Blanchon
bioRxiv 2021.06.28.450131; doi: https://doi.org/10.1101/2021.06.28.450131

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4373)
  • Biochemistry (9561)
  • Bioengineering (7075)
  • Bioinformatics (24800)
  • Biophysics (12581)
  • Cancer Biology (9929)
  • Cell Biology (14306)
  • Clinical Trials (138)
  • Developmental Biology (7938)
  • Ecology (12085)
  • Epidemiology (2067)
  • Evolutionary Biology (15965)
  • Genetics (10910)
  • Genomics (14716)
  • Immunology (9850)
  • Microbiology (23597)
  • Molecular Biology (9463)
  • Neuroscience (50757)
  • Paleontology (369)
  • Pathology (1537)
  • Pharmacology and Toxicology (2675)
  • Physiology (4003)
  • Plant Biology (8646)
  • Scientific Communication and Education (1506)
  • Synthetic Biology (2388)
  • Systems Biology (6417)
  • Zoology (1345)