Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

TMEM120A/TACAN inhibits mechanically activated Piezo2 channels

View ORCID ProfileJohn Smith Del Rosario, Matthew Gabrielle, Yevgen Yudin, View ORCID ProfileTibor Rohacs
doi: https://doi.org/10.1101/2021.06.30.450616
John Smith Del Rosario
Department of Pharmacology, Physiology and Neuroscience, Rutgers, New Jersey Medical School, Newark, NJ 08540
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for John Smith Del Rosario
Matthew Gabrielle
Department of Pharmacology, Physiology and Neuroscience, Rutgers, New Jersey Medical School, Newark, NJ 08540
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yevgen Yudin
Department of Pharmacology, Physiology and Neuroscience, Rutgers, New Jersey Medical School, Newark, NJ 08540
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tibor Rohacs
Department of Pharmacology, Physiology and Neuroscience, Rutgers, New Jersey Medical School, Newark, NJ 08540
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tibor Rohacs
  • For correspondence: tibor.rohacs@rutgers.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

Mechanically activated Piezo2 channels are key mediators of light touch and proprioception in mice and humans. Relatively little is known about what other proteins regulate Piezo2 activity in a cellular context. TACAN (TMEM120A) was proposed to act as a high threshold mechanically activated ion channel in nociceptive dorsal root ganglion (DRG) neurons. Here we find that TACAN co-expression robustly reduced mechanically activated Piezo2 currents, but did not inhibit mechanically activated Piezo1 and TREK1 currents. TACAN co-expression did not affect cell surface expression of either Piezo1 or Piezo2 and did not have major effects on the cortical actin or tubulin cytoskeleton. TACAN expression alone did not result in the appearance of mechanically activated currents above background. In addition, TACAN and Piezo2 expression in DRG neurons overlapped, and siRNA mediated knockdown of TACAN did not decrease the proportion of slowly adapting mechanically activated currents, but resulted in an increased proportion of rapidly adapting currents. Our data do not support TACAN being a mechanically activated ion channel, and identify it as a negative modulator of Piezo2 channel activity.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted June 30, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
TMEM120A/TACAN inhibits mechanically activated Piezo2 channels
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
TMEM120A/TACAN inhibits mechanically activated Piezo2 channels
John Smith Del Rosario, Matthew Gabrielle, Yevgen Yudin, Tibor Rohacs
bioRxiv 2021.06.30.450616; doi: https://doi.org/10.1101/2021.06.30.450616
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
TMEM120A/TACAN inhibits mechanically activated Piezo2 channels
John Smith Del Rosario, Matthew Gabrielle, Yevgen Yudin, Tibor Rohacs
bioRxiv 2021.06.30.450616; doi: https://doi.org/10.1101/2021.06.30.450616

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4667)
  • Biochemistry (10332)
  • Bioengineering (7653)
  • Bioinformatics (26277)
  • Biophysics (13497)
  • Cancer Biology (10663)
  • Cell Biology (15388)
  • Clinical Trials (138)
  • Developmental Biology (8480)
  • Ecology (12800)
  • Epidemiology (2067)
  • Evolutionary Biology (16817)
  • Genetics (11378)
  • Genomics (15451)
  • Immunology (10591)
  • Microbiology (25139)
  • Molecular Biology (10186)
  • Neuroscience (54317)
  • Paleontology (399)
  • Pathology (1663)
  • Pharmacology and Toxicology (2889)
  • Physiology (4331)
  • Plant Biology (9223)
  • Scientific Communication and Education (1585)
  • Synthetic Biology (2551)
  • Systems Biology (6769)
  • Zoology (1459)