Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Viscoelastic Parameterization of Human Skin Cells to Characterize Material Behavior at Multiple Timescales

View ORCID ProfileCameron H. Parvini, View ORCID ProfileAlexander X. Cartagena-Rivera, Santiago D. Solares
doi: https://doi.org/10.1101/2021.07.09.451793
Cameron H. Parvini
1Department of Mechanical and Aerospace Engineering, The George Washington University School of Engineering and Applied Science, Washington, District of Columbia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Cameron H. Parvini
Alexander X. Cartagena-Rivera
2Section on Mechanobiology, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alexander X. Cartagena-Rivera
  • For correspondence: alexander.cartagena-rivera@nih.gov ssolares@gwu.edu
Santiago D. Solares
1Department of Mechanical and Aerospace Engineering, The George Washington University School of Engineering and Applied Science, Washington, District of Columbia, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: alexander.cartagena-rivera@nih.gov ssolares@gwu.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Data/Code
  • Preview PDF
Loading

Abstract

Countless biophysical studies have sought distinct markers in the cellular mechanical response that could be linked to morphogenesis, homeostasis, and disease. Here, a novel iterative-fitting methodology is used to investigate the viscoelastic behavior at multiple relaxation times of human skin cells under physiologically relevant conditions. Past investigations often involved parameterizing linear elastic relationships and assuming purely Hertzian contact mechanics. However, linear elastic treatment fails to capture and properly account for the rich temporal information available in datasets. We demonstrate the performance superiority of the proposed iterative viscoelastic characterization method over standard open-search approaches. Our viscoelastic measurements revealed that 2D adherent metastatic melanoma cells exhibit reduced elasticity compared to normal counterparts—melanocytes and fibroblasts, whereas are significantly less viscous than only fibroblasts over timescales spanning three orders of magnitude. Interestingly, melanocytes are stiffer than melanoma cells, while being the less viscous cells measured. The measured loss angle indicates clear differential viscoelastic responses across multiple timescales between the measured cells. We propose the use of viscoelastic properties at multiple timescales as a mechanical biomarker of diseases. Altogether, this method provides new insight into the complex viscoelastic behavior of metastatic melanoma cells relevant to better understanding cancer metastasis aggression.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Competing Interest Statement: The authors have no competing interests.

  • https://github.com/cparvini/ViscoelasticIterativeExtraction-Cells

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted July 11, 2021.
Download PDF
Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Viscoelastic Parameterization of Human Skin Cells to Characterize Material Behavior at Multiple Timescales
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Viscoelastic Parameterization of Human Skin Cells to Characterize Material Behavior at Multiple Timescales
Cameron H. Parvini, Alexander X. Cartagena-Rivera, Santiago D. Solares
bioRxiv 2021.07.09.451793; doi: https://doi.org/10.1101/2021.07.09.451793
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Viscoelastic Parameterization of Human Skin Cells to Characterize Material Behavior at Multiple Timescales
Cameron H. Parvini, Alexander X. Cartagena-Rivera, Santiago D. Solares
bioRxiv 2021.07.09.451793; doi: https://doi.org/10.1101/2021.07.09.451793

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4397)
  • Biochemistry (9630)
  • Bioengineering (7123)
  • Bioinformatics (24939)
  • Biophysics (12670)
  • Cancer Biology (9995)
  • Cell Biology (14404)
  • Clinical Trials (138)
  • Developmental Biology (7989)
  • Ecology (12147)
  • Epidemiology (2067)
  • Evolutionary Biology (16025)
  • Genetics (10951)
  • Genomics (14778)
  • Immunology (9906)
  • Microbiology (23739)
  • Molecular Biology (9506)
  • Neuroscience (51051)
  • Paleontology (370)
  • Pathology (1545)
  • Pharmacology and Toxicology (2692)
  • Physiology (4038)
  • Plant Biology (8693)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2404)
  • Systems Biology (6459)
  • Zoology (1350)