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Structure of bacterial communities in Japanese-style bathrooms: Comparative sequencing of bacteria in shower water and showerhead biofilms using a portable nanopore sequencer

So Fujiyoshi, Yukiko Nishiuchi, View ORCID ProfileFumito Maruyama
doi: https://doi.org/10.1101/2021.07.14.452346
So Fujiyoshi
aOffice of Industry-Academia-Government and Community Collaboration, Hiroshima University, Hiroshima, Japan
bCenter for Holobiome and Built Environment (CHOBE), Hiroshima University, Hiroshima, Japan
cGraduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
dNetwork for Extreme Environment Research (NEXER), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco, Chile
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  • For correspondence: fujiyoshi.so.62w@kyoto-u.jp
Yukiko Nishiuchi
aOffice of Industry-Academia-Government and Community Collaboration, Hiroshima University, Hiroshima, Japan
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Fumito Maruyama
aOffice of Industry-Academia-Government and Community Collaboration, Hiroshima University, Hiroshima, Japan
bCenter for Holobiome and Built Environment (CHOBE), Hiroshima University, Hiroshima, Japan
cGraduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
dNetwork for Extreme Environment Research (NEXER), Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco, Chile
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  • ORCID record for Fumito Maruyama
  • For correspondence: fumito@hiroshima-u.ac.jp
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Abstract

Showers are one of the main exposure routes to diverse microbes for end users in built environments. Bacteria in water are responsible for biofilm formation on surfaces, and the inside of a showerhead is a specific niche. Here, for the purpose of microbial characterization, source estimation and possibility of infection, the bacterial compositions of both shower water and showerhead biofilms in the same bathroom were determined and compared using a portable nanopore sequencer. The results suggest that specific bacteria in source water would primarily adhere to the surface of the showerhead where they subsequently form biofilms, and the community compositions within biofilms largely vary depending on environmental factors. The relative abundance of several pathogenic bacterial genera in both water and biofilm samples was low. We suggest that it is important to manage risk of infection in each household, and rapid on-site analysis of microbial communities will allow the realization.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 15, 2021.
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Structure of bacterial communities in Japanese-style bathrooms: Comparative sequencing of bacteria in shower water and showerhead biofilms using a portable nanopore sequencer
So Fujiyoshi, Yukiko Nishiuchi, Fumito Maruyama
bioRxiv 2021.07.14.452346; doi: https://doi.org/10.1101/2021.07.14.452346
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Structure of bacterial communities in Japanese-style bathrooms: Comparative sequencing of bacteria in shower water and showerhead biofilms using a portable nanopore sequencer
So Fujiyoshi, Yukiko Nishiuchi, Fumito Maruyama
bioRxiv 2021.07.14.452346; doi: https://doi.org/10.1101/2021.07.14.452346

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