Abstract
Bone repair induced by stem cells and biomaterials may represent an alternative to autologous bone grafting. Here, we compared the efficiency of two biomaterials - biphasic calcium phosphate (BCP) and bioactive glass (BG) - when loaded with either adult bone marrow mesenchymal stem cells (BM-MSCs) or newborn nasal ecto-mesenchymal stem cells (NE-MSCs), the latter being collected for further repair of lip cleft-associated bone loss. Both cell types display the typical stem cell surface markers CD73+/CD90+/CD105+/nestin, and exhibit the MSC-associated osteogenic, chondrogenic and adipogenic multipotency. NE-MSCs produce less collagen and alkaline phosphatase than BM-MSCs. At the transcript level, NE-MSCs express more abundantly three genes coding for bone sialoprotein, osteocalcin and osteopontin, while BM-MSCs produce extra copies of RUNX2. BM-MSCs and NE-MSCs adhere and survive on BCP and BG. In vivo experiments reveal that bone formation is only observed with BM-MSCs transplanted on BCP biomaterial.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
Funding Statement: This work was supported by AORC Junior 2014-2015 grant [grant number NB/JM/1144-14, 2015, recipient LF].
Financial Disclosure Statement: Authors have nothing to disclose.
Presented at: the 73th congress of the French Pediatric Surgery Society (SFCP) 2016 Toulouse France
List of abbreviations
- MSCs
- mesenchymal stromal stem cells
- hMSCs
- human mesenchymal stem cells
- BM-MSCs
- bone marrow derived MSCs
- NE-MSCs
- nasal ecto-mesenchymal stem cells
- GMP
- good manufacturing practice
- BCP
- biphasic calcium phosphate
- HA
- hydroxyapatite
- βTCP
- beta-tricalcium phosphate
- BG
- bioactive glass
- PBS
- phosphate buffered saline
- CPS
- calcium phosphate supersaturated solution
- PFA
- paraformaldehyde
- RTqPCR
- real time quantitative PCR
- GAPDH
- glyceraldehyde 3-phosphate dehydrogenase
- B2M
- beta-2-microglobulin
- RUNX2
- RUNX family transcription factor 2
- BSP
- bone salioprotein
- COLA1
- collagen A1
- OC
- osteocalcin
- BMP2
- bone morphogenic protein 2
- BMP4
- bone morphogenic protein 4
- ALP
- alkaline phosphatase
- OP
- osteopontin
- RT
- room temperature