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Auxin and pectin remodeling interplay during rootlet emergence in white lupin

View ORCID ProfileFrançois Jobert, Alexandre Soriano, View ORCID ProfileLaurent Brottier, Célia Casset, Fanchon Divol, View ORCID ProfileJosip Safran, View ORCID ProfileValérie Lefebvre, View ORCID ProfileJérôme Pelloux, View ORCID ProfileStéphanie Robert, View ORCID ProfileBenjamin Péret
doi: https://doi.org/10.1101/2021.07.19.452882
François Jobert
1BPMP, Univ Montpellier, CNRS, INRAE, Supagro, Montpellier, France
2Umeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden
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Alexandre Soriano
1BPMP, Univ Montpellier, CNRS, INRAE, Supagro, Montpellier, France
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Laurent Brottier
1BPMP, Univ Montpellier, CNRS, INRAE, Supagro, Montpellier, France
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Célia Casset
1BPMP, Univ Montpellier, CNRS, INRAE, Supagro, Montpellier, France
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Fanchon Divol
1BPMP, Univ Montpellier, CNRS, INRAE, Supagro, Montpellier, France
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Josip Safran
3UMR INRAE 1158 BioEcoAgro, BIOPI Biologie des Plantes et Innovation, SFR Condorcet FR CNRS 3417, Université de Picardie, Amiens, France
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Valérie Lefebvre
3UMR INRAE 1158 BioEcoAgro, BIOPI Biologie des Plantes et Innovation, SFR Condorcet FR CNRS 3417, Université de Picardie, Amiens, France
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Jérôme Pelloux
3UMR INRAE 1158 BioEcoAgro, BIOPI Biologie des Plantes et Innovation, SFR Condorcet FR CNRS 3417, Université de Picardie, Amiens, France
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Stéphanie Robert
2Umeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden
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  • For correspondence: stephanie.robert@slu.se benjamin.peret@cnrs.fr
Benjamin Péret
1BPMP, Univ Montpellier, CNRS, INRAE, Supagro, Montpellier, France
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  • For correspondence: stephanie.robert@slu.se benjamin.peret@cnrs.fr
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Abstract

Secondary root emergence is a crucial trait that shapes the plant’s underground system. Virtually every developmental step of root primordium morphogenesis is controlled by auxin. However, how the hormone controls cell separation in primordium-overlaying tissues through wall loosening is poorly understood. Here, we took advantage of white lupin and its spectacular cluster root development to assess the contribution of auxin to this process. We show that auxin’s positive role on rootlet emergence is associated with an upregulation of cell wall pectin modifying and degrading genes. Downregulation of a pectinolytic enzyme gene expressed in cells surrounding the primordium resulted in delayed emergence. Pectins were demethylesterified in the emergence zone and auxin treatment further enhanced this effect. Additionally, we report specific rhamnogalacturonan-I modifications during cortical cell separation. In conclusion, we propose a model in which auxin has a dual role during rootlet emergence: Firstly, through active pectin demethylesterification and secondly by regulating the expression of cell wall remodeling enzymes.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 19, 2021.
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Auxin and pectin remodeling interplay during rootlet emergence in white lupin
François Jobert, Alexandre Soriano, Laurent Brottier, Célia Casset, Fanchon Divol, Josip Safran, Valérie Lefebvre, Jérôme Pelloux, Stéphanie Robert, Benjamin Péret
bioRxiv 2021.07.19.452882; doi: https://doi.org/10.1101/2021.07.19.452882
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Auxin and pectin remodeling interplay during rootlet emergence in white lupin
François Jobert, Alexandre Soriano, Laurent Brottier, Célia Casset, Fanchon Divol, Josip Safran, Valérie Lefebvre, Jérôme Pelloux, Stéphanie Robert, Benjamin Péret
bioRxiv 2021.07.19.452882; doi: https://doi.org/10.1101/2021.07.19.452882

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