Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Growth and immunolocalisation of the brown alga Ectocarpus in a microfluidic environment

View ORCID ProfileBénédicte Charrier, Samuel Boscq, Bradley J. Nelson, Nino F. Läubli
doi: https://doi.org/10.1101/2021.07.20.453111
Bénédicte Charrier
1Modeling and Morphogenesis of Macroalgae, UMR8227, CNRS - Sorbonne University, Marine Biological Station, Roscoff, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Bénédicte Charrier
  • For correspondence: benedicte.charrier@sb-roscoff.fr laeublin@ethz.ch
Samuel Boscq
1Modeling and Morphogenesis of Macroalgae, UMR8227, CNRS - Sorbonne University, Marine Biological Station, Roscoff, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bradley J. Nelson
2Multi-Scale Robotics Lab, ETH Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nino F. Läubli
2Multi-Scale Robotics Lab, ETH Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: benedicte.charrier@sb-roscoff.fr laeublin@ethz.ch
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

PDMS chips have proven to be suitable environments for the growth of several filamentous organisms. However, depending on the specimen, the pattern of growth and cell differentiation has been rarely investigated. We monitored the developmental pattern of the brown alga Ectocarpus inside a PDMS lab-on-chip. Two main methods of inoculation of the lab-on-chip were tested, i.e. by injection of spores or by insertion of sporophyte filaments into the chamber. Growth rate, growth trajectory, cell differentiation, and branching were the main development steps that were monitored for 20 days inside 25 μm or 40 μm parallel channels under standard light and temperature conditions. They were shown to be similar to those observed in non-constrained in-vitro conditions. Labelling of Ectocarpus cell wall polysaccharides – both with calcofluor for cellulose, and by immunolocalisation for alginates with monoclonal antibodies–showed expected patterns when compared to open space growth using either epifluorescence or confocal microscopy. Overall this article describes the experimental conditions for observing and studying the basic unaltered processes of brown algal growth using microfluidic technology, which provides the basis for future biochemical and biological research.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted July 20, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Growth and immunolocalisation of the brown alga Ectocarpus in a microfluidic environment
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Growth and immunolocalisation of the brown alga Ectocarpus in a microfluidic environment
Bénédicte Charrier, Samuel Boscq, Bradley J. Nelson, Nino F. Läubli
bioRxiv 2021.07.20.453111; doi: https://doi.org/10.1101/2021.07.20.453111
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Growth and immunolocalisation of the brown alga Ectocarpus in a microfluidic environment
Bénédicte Charrier, Samuel Boscq, Bradley J. Nelson, Nino F. Läubli
bioRxiv 2021.07.20.453111; doi: https://doi.org/10.1101/2021.07.20.453111

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4838)
  • Biochemistry (10738)
  • Bioengineering (8016)
  • Bioinformatics (27182)
  • Biophysics (13939)
  • Cancer Biology (11083)
  • Cell Biology (15987)
  • Clinical Trials (138)
  • Developmental Biology (8758)
  • Ecology (13238)
  • Epidemiology (2067)
  • Evolutionary Biology (17316)
  • Genetics (11665)
  • Genomics (15885)
  • Immunology (10991)
  • Microbiology (25995)
  • Molecular Biology (10608)
  • Neuroscience (56354)
  • Paleontology (417)
  • Pathology (1728)
  • Pharmacology and Toxicology (2999)
  • Physiology (4530)
  • Plant Biology (9590)
  • Scientific Communication and Education (1610)
  • Synthetic Biology (2671)
  • Systems Biology (6960)
  • Zoology (1507)