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Beneficial effects of novel strains of Aureobasidium pullulans produced 1,3-1,6 β-glucans on non-esterified fatty acid levels in diabetic KKAy mice

Nobunao Ikewaki, Takashi Onaka, Yasunori Ikeue, Mitsuru Nagataki, Gene Kurosawa, Vidyasagar Devaprasad Dedeepiya, Mathaiyan Rajmohan, Suryaprakash Vaddi, Rajappa Senthilkumar, Senthilkumar Preethy, View ORCID ProfileSamuel JK Abraham
doi: https://doi.org/10.1101/2021.07.22.453362
Nobunao Ikewaki
1Dept. of Medical Life Science, Kyushu University of Health and Welfare, Japan
2Institute of Immunology, Junsei Educational Institute, Nobeoka, Miyazaki, Japan
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Takashi Onaka
3Sophy Inc., Kochi, Japan
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Yasunori Ikeue
3Sophy Inc., Kochi, Japan
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Mitsuru Nagataki
3Sophy Inc., Kochi, Japan
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Gene Kurosawa
4Department of Academic Research Support Promotion Facility, Center for Research Promotion and Support, Fujita Health University, Aichi, Japan
5MabGenesis KK, Nagoya, Japan
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Vidyasagar Devaprasad Dedeepiya
6Mary-Yoshio Translational Hexagon (MYTH), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India
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Mathaiyan Rajmohan
7Fujio-Eiji Academic Terrain (FEAT), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India
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Suryaprakash Vaddi
8Department of Urology, Yashoda Hospitals, Hyderabad, India
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Rajappa Senthilkumar
7Fujio-Eiji Academic Terrain (FEAT), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India
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Senthilkumar Preethy
7Fujio-Eiji Academic Terrain (FEAT), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India
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Samuel JK Abraham
6Mary-Yoshio Translational Hexagon (MYTH), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India
9Centre for Advancing Clinical Research (CACR), University of Yamanashi - School of Medicine, Chuo, Japan
10Antony- Xavier Interdisciplinary Scholastics (AXIS), GN Corporation Co. Ltd., Kofu, Japan
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  • ORCID record for Samuel JK Abraham
  • For correspondence: drsam@nichimail.jp drspp@nichimail.jp
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Abstract

Obesity, metabolic syndrome, associated lipotoxicity and its cascade of events contribute to the majority of the burden related to non-communicable diseases globally. Preventive lifestyle changes aside, several beneficial effects have been reported in type II diabetes mellitus and dyslipidaemia patients with biological response modifier glucans (BRMG) produced as an exopolysaccharide by Aureobasidium pullulans. In this study, we compared two strains (AFO-202 and N-163) that produce beta glucans in alleviating lipotoxicity. This study was performed in obese diabetic mice model of KK-Ay mice, in four groups with six subjects in each group - Group 1: sacrificed on Day 0 for baseline values; Group 2: control (drinking water); Group 3: AFO-202 beta glucan—200 mg/kg/day; Group 4: N-163 beta glucan—300 mg/kg/day. The animals in groups 2–4 had the test solutions administered by gavage once daily for 28 consecutive days. Biochemical analyses were conducted of blood glucose, triglycerides, total cholesterol, LDL cholesterol, HDL cholesterol and non-esterified fatty acids (NEFA). Group 4 (N-163) had the lowest NEFA levels, as compared to the other groups, and marginally decreased triglyceride levels. The groups had no significant differences in blood glucose, HbA1c, triglycerides, or LDL and HDL cholesterol. N-163 produced by A. pullulans decreased NEFA in a diabetic mice model in 28 days. These results, although modest, warrant further in-depth research into lipotoxicity and associated inflammatory cascades in both healthy and disease affected subjects to develop novel strategies for prevention and management.

Competing Interest Statement

1.Author Samuel Abraham is a shareholder in GN Corporation, Japan which in turn is a shareholder in the manufacturing company of the Beta Glucans described in the study. 2.Author Takashi Onaka is a shareholder and Yasunori Ikeue is member of the board in the manufacturing company of the Beta Glucans described in the study.

Footnotes

  • (nikewaki{at}phoenix.ac.jp)

  • (onaka-t{at}sophy-inc.co.jp)

  • (ikeue-y{at}sophy-inc.co.jp)

  • (nagataki-m{at}sophy-inc.co.jp)

  • (gene{at}fujita-hu.ac.jp)

  • (dedeepiya_76{at}yahoo.co.in)

  • (mrm{at}nichimail.jp)

  • (suryaprakashuro{at}gmail.com)

  • (rsk{at}nichimail.jp)

  • (drspp{at}nichimail.jp)

  • (drsam{at}nichimail.jp)

  • Funding: No external funding was received for the study

  • Potential Conflict of Interests: 1. Author Samuel Abraham is a shareholder in GN Corporation, Japan which in turn is a shareholder in the manufacturing company of the Beta Glucans described in the study.

  • 2. Author Takashi Onaka is a shareholder and Yasunori Ikeue is member of the board in the manufacturing company of the Beta Glucans described in the study.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted August 03, 2021.
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Beneficial effects of novel strains of Aureobasidium pullulans produced 1,3-1,6 β-glucans on non-esterified fatty acid levels in diabetic KKAy mice
Nobunao Ikewaki, Takashi Onaka, Yasunori Ikeue, Mitsuru Nagataki, Gene Kurosawa, Vidyasagar Devaprasad Dedeepiya, Mathaiyan Rajmohan, Suryaprakash Vaddi, Rajappa Senthilkumar, Senthilkumar Preethy, Samuel JK Abraham
bioRxiv 2021.07.22.453362; doi: https://doi.org/10.1101/2021.07.22.453362
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Beneficial effects of novel strains of Aureobasidium pullulans produced 1,3-1,6 β-glucans on non-esterified fatty acid levels in diabetic KKAy mice
Nobunao Ikewaki, Takashi Onaka, Yasunori Ikeue, Mitsuru Nagataki, Gene Kurosawa, Vidyasagar Devaprasad Dedeepiya, Mathaiyan Rajmohan, Suryaprakash Vaddi, Rajappa Senthilkumar, Senthilkumar Preethy, Samuel JK Abraham
bioRxiv 2021.07.22.453362; doi: https://doi.org/10.1101/2021.07.22.453362

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