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Polymer-Conjugated Carbon Nanotubes for Biomolecule Loading

View ORCID ProfileChristopher T. Jackson, Jeffrey W. Wang, View ORCID ProfileEduardo González-Grandío, Natalie S. Goh, Jaewan Mun, Sejal Krishnan, Markita P. Landry
doi: https://doi.org/10.1101/2021.07.22.453422
Christopher T. Jackson
1Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA
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Jeffrey W. Wang
1Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA
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Eduardo González-Grandío
1Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA
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Natalie S. Goh
1Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA
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Jaewan Mun
1Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA
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Sejal Krishnan
1Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA
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Markita P. Landry
1Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA
2Innovative Genomics Institute (IGI), Berkeley, CA, USA
3California Institute for Quantitative Biosciences, QB3, University of California, Berkeley, CA, USA
4Chan-Zuckerberg Biohub, San Francisco, CA, USA
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  • For correspondence: landry@berkeley.edu
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Abstract

Nanomaterials have emerged as an invaluable tool for the delivery of biomolecules such as DNA and RNA, with various applications in genetic engineering and post-transcriptional genetic manipulation. Alongside this development, there has been an increasing use of polymer-based techniques, such as polyethyleneimine (PEI), to electrostatically load polynucleotide cargoes onto nanomaterial carriers. However, there remains a need to assess nanomaterial properties, conjugation conditions, and biocompatibility of these nanomaterial-polymer constructs, particularly for use in plant systems. In this work, we develop mechanisms to optimize DNA loading on single-walled carbon nanotubes (SWNTs) with a library of polymer-SWNT constructs and assess DNA loading ability, polydispersity, and both chemical and colloidal stability. Counterintuitively, we demonstrate that polymer hydrolysis from nanomaterial surfaces can occur depending on polymer properties and attachment chemistries, and describe mitigation strategies against construct degradation. Given the growing interest in delivery applications in plant systems, we also assess the toxicity of polymer-based nanomaterials in plants and provide recommendations for future design of nanomaterial-based polynucleotide delivery strategies.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 23, 2021.
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Polymer-Conjugated Carbon Nanotubes for Biomolecule Loading
Christopher T. Jackson, Jeffrey W. Wang, Eduardo González-Grandío, Natalie S. Goh, Jaewan Mun, Sejal Krishnan, Markita P. Landry
bioRxiv 2021.07.22.453422; doi: https://doi.org/10.1101/2021.07.22.453422
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Polymer-Conjugated Carbon Nanotubes for Biomolecule Loading
Christopher T. Jackson, Jeffrey W. Wang, Eduardo González-Grandío, Natalie S. Goh, Jaewan Mun, Sejal Krishnan, Markita P. Landry
bioRxiv 2021.07.22.453422; doi: https://doi.org/10.1101/2021.07.22.453422

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