New Results
Genomic rearrangements have consequences for introgression breeding as revealed by genome assemblies of wild and cultivated lentil species
Larissa Ramsay, View ORCID ProfileChu Shin Koh, View ORCID ProfileSateesh Kagale, Dongying Gao, Sukhjiwan Kaur, View ORCID ProfileTeketel Haile, Tadesse S. Gela, Li-An Chen, Zhe Cao, David J. Konkin, Helena Toegelová, Jaroslav Doležel, Benjamin D. Rosen, Robert Stonehouse, View ORCID ProfileJodi L. Humann, View ORCID ProfileDorrie Main, View ORCID ProfileClarice J. Coyne, Rebecca J. McGee, Douglas R. Cook, View ORCID ProfileR. Varma Penmetsa, View ORCID ProfileAlbert Vandenberg, Crystal Chan, View ORCID ProfileSabine Banniza, David Edwards, View ORCID ProfilePhilipp E. Bayer, View ORCID ProfileJacqueline Batley, View ORCID ProfileSripada M. Udupa, View ORCID ProfileKirstin E. Bett
doi: https://doi.org/10.1101/2021.07.23.453237
Larissa Ramsay
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
Chu Shin Koh
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
Sateesh Kagale
2National Research Council of Canada Saskatoon, Canada
Dongying Gao
3USDA-ARS, Small Grains and Potato Germplasm Research Unit, Aberdeen, ID, USA
Sukhjiwan Kaur
4Agriculture Victoria, Department of Jobs, Precincts and Regions, AgriBio, Centre for AgriBioscience, Bundoora, Victoria, Australia
Teketel Haile
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
Tadesse S. Gela
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
Li-An Chen
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
Zhe Cao
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
David J. Konkin
2National Research Council of Canada Saskatoon, Canada
Helena Toegelová
5Institute of Experimental Botany of the Czech Academy of Sciences, Center of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic
Jaroslav Doležel
5Institute of Experimental Botany of the Czech Academy of Sciences, Center of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic
Benjamin D. Rosen
6USDA, ARS, Animal Genomics and Improvement Laboratory, Beltsville, MD, USA.
Robert Stonehouse
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
Jodi L. Humann
7Department of Horticulture, Washington State University, Pullman WA, USA.
Dorrie Main
7Department of Horticulture, Washington State University, Pullman WA, USA.
Clarice J. Coyne
8USDA, ARS, Plant Germplasm Introduction and Testing Research, Pullman, WA, USA
Rebecca J. McGee
9USDA, ARS, Grain Legume Genetics and Physiology Research Unit, Pullman, WA
Douglas R. Cook
10Department of Plant Pathology, University of California Davis, Davis CA, USA
R. Varma Penmetsa
10Department of Plant Pathology, University of California Davis, Davis CA, USA
Albert Vandenberg
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
Crystal Chan
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
Sabine Banniza
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
David Edwards
11School of Biological Sciences and School of Agriculture, University of Western Australia, Crawley, WA, Australia
Philipp E. Bayer
11School of Biological Sciences and School of Agriculture, University of Western Australia, Crawley, WA, Australia
Jacqueline Batley
11School of Biological Sciences and School of Agriculture, University of Western Australia, Crawley, WA, Australia
Sripada M. Udupa
12International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco
Kirstin E. Bett
1Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada
Abstract
Understanding the genomic relationship between wild and cultivated genomes would facilitate access to the untapped variability found in crop wild relatives. We developed genome assemblies of a cultivated lentil (Lens culinaris) as well as a wild relative (L. ervoides). Comparative analyses revealed large-scale structural rearrangements and additional repetitive DNA in the cultivated genome, resulting in regions of reduced recombination, segregation distortion and permanent heterozygosity in the offspring of a cross between the two species. These novel findings provide plant breeders with better insight into how best to approach accessing the novel variability available in wild relatives.
Competing Interest Statement
The authors have declared no competing interest.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted July 24, 2021.
Genomic rearrangements have consequences for introgression breeding as revealed by genome assemblies of wild and cultivated lentil species
Larissa Ramsay, Chu Shin Koh, Sateesh Kagale, Dongying Gao, Sukhjiwan Kaur, Teketel Haile, Tadesse S. Gela, Li-An Chen, Zhe Cao, David J. Konkin, Helena Toegelová, Jaroslav Doležel, Benjamin D. Rosen, Robert Stonehouse, Jodi L. Humann, Dorrie Main, Clarice J. Coyne, Rebecca J. McGee, Douglas R. Cook, R. Varma Penmetsa, Albert Vandenberg, Crystal Chan, Sabine Banniza, David Edwards, Philipp E. Bayer, Jacqueline Batley, Sripada M. Udupa, Kirstin E. Bett
bioRxiv 2021.07.23.453237; doi: https://doi.org/10.1101/2021.07.23.453237
Genomic rearrangements have consequences for introgression breeding as revealed by genome assemblies of wild and cultivated lentil species
Larissa Ramsay, Chu Shin Koh, Sateesh Kagale, Dongying Gao, Sukhjiwan Kaur, Teketel Haile, Tadesse S. Gela, Li-An Chen, Zhe Cao, David J. Konkin, Helena Toegelová, Jaroslav Doležel, Benjamin D. Rosen, Robert Stonehouse, Jodi L. Humann, Dorrie Main, Clarice J. Coyne, Rebecca J. McGee, Douglas R. Cook, R. Varma Penmetsa, Albert Vandenberg, Crystal Chan, Sabine Banniza, David Edwards, Philipp E. Bayer, Jacqueline Batley, Sripada M. Udupa, Kirstin E. Bett
bioRxiv 2021.07.23.453237; doi: https://doi.org/10.1101/2021.07.23.453237
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