Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

CardiOvascular examination in awake Orangutans (Pongo pygmaeus pygmaeus): Low-stress Echocardiography including Speckle Tracking imaging (the COOLEST method)

View ORCID ProfileValérie Chetboul, Didier Concordet, Renaud Tissier, Irène Vonfeld, Camille Poissonnier, Maria Paz Alvarado, Peggy Passavin, Mathilde Gluntz, Solène Lefort, Aude Bourgeois, Dylan Duby, Christelle Hano, Norin Chai
doi: https://doi.org/10.1101/2021.07.23.453539
Valérie Chetboul
1École nationale vétérinaire d’Alfort, CHUVA, Unité de Cardiologie d’Alfort (UCA), F-94700 Maisons-Alfort, France
2Univ Paris Est Créteil, INSERM, IMRB, F-94010 Créteil, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Valérie Chetboul
  • For correspondence: valerie.chetboul@vet-alfort.fr
Didier Concordet
3Intheres, Université de Toulouse, INRA, ENVT, Toulouse Cedex 3, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Renaud Tissier
2Univ Paris Est Créteil, INSERM, IMRB, F-94010 Créteil, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Irène Vonfeld
1École nationale vétérinaire d’Alfort, CHUVA, Unité de Cardiologie d’Alfort (UCA), F-94700 Maisons-Alfort, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Camille Poissonnier
1École nationale vétérinaire d’Alfort, CHUVA, Unité de Cardiologie d’Alfort (UCA), F-94700 Maisons-Alfort, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maria Paz Alvarado
1École nationale vétérinaire d’Alfort, CHUVA, Unité de Cardiologie d’Alfort (UCA), F-94700 Maisons-Alfort, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peggy Passavin
1École nationale vétérinaire d’Alfort, CHUVA, Unité de Cardiologie d’Alfort (UCA), F-94700 Maisons-Alfort, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mathilde Gluntz
1École nationale vétérinaire d’Alfort, CHUVA, Unité de Cardiologie d’Alfort (UCA), F-94700 Maisons-Alfort, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Solène Lefort
1École nationale vétérinaire d’Alfort, CHUVA, Unité de Cardiologie d’Alfort (UCA), F-94700 Maisons-Alfort, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aude Bourgeois
4Ménagerie, le Zoo du Jardin des Plantes, Muséum National d’Histoire Naturelle, 75005 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dylan Duby
4Ménagerie, le Zoo du Jardin des Plantes, Muséum National d’Histoire Naturelle, 75005 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christelle Hano
4Ménagerie, le Zoo du Jardin des Plantes, Muséum National d’Histoire Naturelle, 75005 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Norin Chai
4Ménagerie, le Zoo du Jardin des Plantes, Muséum National d’Histoire Naturelle, 75005 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Introduction Cardiovascular diseases have been identified as a major cause of mortality and morbidity in Borneo orangutans (Pongo pygmaeus pygmaeus). Transthoracic echocardiography is usually performed under anesthesia in great apes, which may be stressful and risky in cardiac animals. The aim of the present pilot study was hence to develop a quick and non-stressful echocardiographic method (i.e., the COOLEST method) in awake Borneo orangutans (CardiOvascular examination in awake Orangutans: Low-stress Echocardiography including Speckle Tracking imaging) and assess the variability of corresponding variables.

Materials and Methods Four adult Borneo orangutans trained to present their chest to the trainers were involved. A total of 96 TTE examinations were performed on 4 different days by a trained observer examining each orangutans 6 times per day. Each examination included four two-dimensional views, with offline assessment of 28 variables (i.e., two-dimensional (n=12), M-mode and anatomic M-mode (n=6), Doppler (n=7), and speckle tracking imaging (n=3)), representing a total of 2,688 measurements. A general linear model was used to determine the within-day and between-day coefficients of variation.

Results Mean±SD (minimum-maximum) images acquisition duration was 3.8±1.6 minutes (1.3-6.3). All within-day and between-day coefficients of variation but one (n=55/56, 98%) were <15%, and most (51/56, 91%) were <10% including those of speckle tracking systolic strain variables (2.7% to 5.4%).

Discussion Heart morphology as well as global and regional myocardial function can be assessed in awake orangutans with good to excellent repeatability and reproducibility.

Conclusions This non-stressful method may be used for longitudinal cardiac follow-up in awake orangutans.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • This study was not supported by any grant or other funding.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted July 23, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
CardiOvascular examination in awake Orangutans (Pongo pygmaeus pygmaeus): Low-stress Echocardiography including Speckle Tracking imaging (the COOLEST method)
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
CardiOvascular examination in awake Orangutans (Pongo pygmaeus pygmaeus): Low-stress Echocardiography including Speckle Tracking imaging (the COOLEST method)
Valérie Chetboul, Didier Concordet, Renaud Tissier, Irène Vonfeld, Camille Poissonnier, Maria Paz Alvarado, Peggy Passavin, Mathilde Gluntz, Solène Lefort, Aude Bourgeois, Dylan Duby, Christelle Hano, Norin Chai
bioRxiv 2021.07.23.453539; doi: https://doi.org/10.1101/2021.07.23.453539
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
CardiOvascular examination in awake Orangutans (Pongo pygmaeus pygmaeus): Low-stress Echocardiography including Speckle Tracking imaging (the COOLEST method)
Valérie Chetboul, Didier Concordet, Renaud Tissier, Irène Vonfeld, Camille Poissonnier, Maria Paz Alvarado, Peggy Passavin, Mathilde Gluntz, Solène Lefort, Aude Bourgeois, Dylan Duby, Christelle Hano, Norin Chai
bioRxiv 2021.07.23.453539; doi: https://doi.org/10.1101/2021.07.23.453539

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Zoology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3688)
  • Biochemistry (7783)
  • Bioengineering (5673)
  • Bioinformatics (21267)
  • Biophysics (10574)
  • Cancer Biology (8170)
  • Cell Biology (11929)
  • Clinical Trials (138)
  • Developmental Biology (6757)
  • Ecology (10394)
  • Epidemiology (2065)
  • Evolutionary Biology (13853)
  • Genetics (9702)
  • Genomics (13063)
  • Immunology (8136)
  • Microbiology (19976)
  • Molecular Biology (7841)
  • Neuroscience (43032)
  • Paleontology (318)
  • Pathology (1278)
  • Pharmacology and Toxicology (2258)
  • Physiology (3350)
  • Plant Biology (7221)
  • Scientific Communication and Education (1311)
  • Synthetic Biology (2000)
  • Systems Biology (5533)
  • Zoology (1127)