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Distinct mechanisms underlie H2O2 sensing in C. elegans head and tail

View ORCID ProfileSophie Quintin, Théo Aspert, Tao Ye, View ORCID ProfileGilles Charvin
doi: https://doi.org/10.1101/2021.07.26.451501
Sophie Quintin
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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  • For correspondence: quintin@igbmc.fr
Théo Aspert
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Tao Ye
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Gilles Charvin
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Abstract

Environmental oxidative stress threatens cellular integrity and should therefore be avoided by living organisms. Yet, relatively little is known about environmental oxidative stress perception. Here, using microfluidics, we showed that like I2 pharyngeal neurons, the tail phasmid PHA neurons function as oxidative stress sensing neurons in C. elegans, but display different responses to H2O2 and light. We uncovered that different but related receptors, GUR-3 and LITE-1, mediate H2O2 signaling in I2 and PHA neurons. Still, the peroxiredoxin PRDX-2 is essential for both, and might promote H2O2-mediated receptor activation. Our work demonstrates that C. elegans can sense a broad range of oxidative stressors using partially distinct H2O2 signaling pathways in head and tail sensillae, and paves the way for further understanding of how the integration of these inputs translates into the appropriate behavior.

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Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • This revised version includes more data (sek-1/MAPKK mutant analyses), which strengthened our conclusion that the p38MAPK pathway is required in PHA neurons for sensitivity to low doses of H2O2. In addtition, we re-did all statistical analyses, taking into account the multiple comparisons problem. Importantly, performing these more stringent statistical analyses did not change our conclusions. All figures have been updated, and supplementary material includes 4 new movies and 2 new figures.

  • https://github.com/gcharvin/viewworm

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 11, 2022.
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Distinct mechanisms underlie H2O2 sensing in C. elegans head and tail
Sophie Quintin, Théo Aspert, Tao Ye, Gilles Charvin
bioRxiv 2021.07.26.451501; doi: https://doi.org/10.1101/2021.07.26.451501
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Distinct mechanisms underlie H2O2 sensing in C. elegans head and tail
Sophie Quintin, Théo Aspert, Tao Ye, Gilles Charvin
bioRxiv 2021.07.26.451501; doi: https://doi.org/10.1101/2021.07.26.451501

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