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A clinically validated human saliva metatranscriptomic test for global systems biology studies

View ORCID ProfileRyan Toma, Ying Cai, Oyetunji Ogundijo, Stephanie Gline, Diana Demusaj, Nathan Duval, Pedro Torres, Francine Camacho, Guruduth Banavar, Momchilo Vuyisich
doi: https://doi.org/10.1101/2021.08.03.454950
Ryan Toma
1Viome, Inc., Bothell and Bellevue, WA/New York, NY, USA,
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  • For correspondence: ryan.toma@viome.com
Ying Cai
1Viome, Inc., Bothell and Bellevue, WA/New York, NY, USA,
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Oyetunji Ogundijo
1Viome, Inc., Bothell and Bellevue, WA/New York, NY, USA,
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Stephanie Gline
1Viome, Inc., Bothell and Bellevue, WA/New York, NY, USA,
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Diana Demusaj
1Viome, Inc., Bothell and Bellevue, WA/New York, NY, USA,
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Nathan Duval
1Viome, Inc., Bothell and Bellevue, WA/New York, NY, USA,
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Pedro Torres
1Viome, Inc., Bothell and Bellevue, WA/New York, NY, USA,
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Francine Camacho
1Viome, Inc., Bothell and Bellevue, WA/New York, NY, USA,
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Guruduth Banavar
1Viome, Inc., Bothell and Bellevue, WA/New York, NY, USA,
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Momchilo Vuyisich
1Viome, Inc., Bothell and Bellevue, WA/New York, NY, USA,
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Abstract

We report here the development of a high throughput, automated, inexpensive, and clinically validated saliva metatranscriptome test that requires less than 100 microliters of saliva. The RNA in the samples is preserved at the time of collection. Samples can then be stored and transported at ambient temperatures for up to 28 days without significantly affecting the test results. Another important feature of the sample preservative is that it inactivates all classes of pathogens, enabling safe transportation globally. Given the unique set of convenience, low cost, safety, and technical performance, this saliva metatranscriptomic test can be integrated into longitudinal, global scale, systems biology studies that will lead to an accelerated development of precision medicine diagnostic and therapeutic tools.

Method Summary This report describes an important improvement to saliva transcriptome analysis. While current methods are complicated and expensive, the method reported here includes at-home sample collection, global shipping at ambient temperatures and pathogen inactivation at the point of collection; it uses fully automated, clinically validated and licensed laboratory and bioinformatic analyses.

Competing Interest Statement

All authors are current or former employees of Viome, Inc. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

Footnotes

  • Pedro Torres and Francine Camacho added as authors

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted August 06, 2021.
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A clinically validated human saliva metatranscriptomic test for global systems biology studies
Ryan Toma, Ying Cai, Oyetunji Ogundijo, Stephanie Gline, Diana Demusaj, Nathan Duval, Pedro Torres, Francine Camacho, Guruduth Banavar, Momchilo Vuyisich
bioRxiv 2021.08.03.454950; doi: https://doi.org/10.1101/2021.08.03.454950
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A clinically validated human saliva metatranscriptomic test for global systems biology studies
Ryan Toma, Ying Cai, Oyetunji Ogundijo, Stephanie Gline, Diana Demusaj, Nathan Duval, Pedro Torres, Francine Camacho, Guruduth Banavar, Momchilo Vuyisich
bioRxiv 2021.08.03.454950; doi: https://doi.org/10.1101/2021.08.03.454950

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