Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Local regulation of extracellular vesicle traffic by the synaptic endocytic machinery

Cassandra R. Blanchette, Amy L. Scalera, Kathryn P. Harris, Zechuan Zhao, Kate Koles, Anna Yeh, Julia K. Apiki, Bryan A. Stewart, View ORCID ProfileAvital A. Rodal
doi: https://doi.org/10.1101/2021.08.04.454987
Cassandra R. Blanchette
1Department of Biology, Brandeis University, Waltham, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amy L. Scalera
1Department of Biology, Brandeis University, Waltham, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kathryn P. Harris
2Department of Biology, University of Toronto Mississauga, Mississauga, Canada
3Department of Cell and Systems Biology University of Toronto, Toronto, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zechuan Zhao
1Department of Biology, Brandeis University, Waltham, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kate Koles
1Department of Biology, Brandeis University, Waltham, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anna Yeh
1Department of Biology, Brandeis University, Waltham, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julia K. Apiki
1Department of Biology, Brandeis University, Waltham, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bryan A. Stewart
2Department of Biology, University of Toronto Mississauga, Mississauga, Canada
3Department of Cell and Systems Biology University of Toronto, Toronto, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Avital A. Rodal
1Department of Biology, Brandeis University, Waltham, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Avital A. Rodal
  • For correspondence: arodal@brandeis.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Neuronal extracellular vesicles (EVs) carry cargoes that are important in intercellular signaling and disease, but how and where cargoes are sorted into EVs remains unclear. Here, we identified a new role for canonical clathrin-mediated endocytic machinery in controlling EV cargo traffic in Drosophila neurons. Endocytic mutants, including nervous wreck (nwk), Shibire/Dynamin, and AP-2, exhibit local depletion of multiple cargoes in presynaptic EV donor terminals as well as in EVs. Accordingly, nwk mutants phenocopy synaptic plasticity defects associated with loss of the EV cargo Synaptotagmin-4, and suppress lethality upon overexpression of the EV cargo Amyloid Precursor Protein. These EV defects are genetically separable from canonical functions of endocytic proteins in synaptic vesicle recycling and synaptic growth. Nwk opposes the endosomal retromer complex to regulate EV cargo levels, and acts upstream of dynactin-mediated retrograde axonal transport. Our data suggest a novel molecular mechanism that protects EV cargoes from local depletion at synapses.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted August 05, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Local regulation of extracellular vesicle traffic by the synaptic endocytic machinery
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Local regulation of extracellular vesicle traffic by the synaptic endocytic machinery
Cassandra R. Blanchette, Amy L. Scalera, Kathryn P. Harris, Zechuan Zhao, Kate Koles, Anna Yeh, Julia K. Apiki, Bryan A. Stewart, Avital A. Rodal
bioRxiv 2021.08.04.454987; doi: https://doi.org/10.1101/2021.08.04.454987
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Local regulation of extracellular vesicle traffic by the synaptic endocytic machinery
Cassandra R. Blanchette, Amy L. Scalera, Kathryn P. Harris, Zechuan Zhao, Kate Koles, Anna Yeh, Julia K. Apiki, Bryan A. Stewart, Avital A. Rodal
bioRxiv 2021.08.04.454987; doi: https://doi.org/10.1101/2021.08.04.454987

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4116)
  • Biochemistry (8820)
  • Bioengineering (6523)
  • Bioinformatics (23470)
  • Biophysics (11798)
  • Cancer Biology (9216)
  • Cell Biology (13327)
  • Clinical Trials (138)
  • Developmental Biology (7440)
  • Ecology (11417)
  • Epidemiology (2066)
  • Evolutionary Biology (15160)
  • Genetics (10442)
  • Genomics (14051)
  • Immunology (9176)
  • Microbiology (22170)
  • Molecular Biology (8817)
  • Neuroscience (47600)
  • Paleontology (350)
  • Pathology (1429)
  • Pharmacology and Toxicology (2492)
  • Physiology (3733)
  • Plant Biology (8084)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2221)
  • Systems Biology (6039)
  • Zoology (1254)