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A toolbox for studying cortical physiology in primates

View ORCID ProfileKaram Khateeb, View ORCID ProfileJulien Bloch, View ORCID ProfileJasmine Zhou, Mona Rahimi, Devon J. Griggs, View ORCID ProfileViktor N. Kharazia, Min N. Le, Ruikang Wang, View ORCID ProfileAzadeh Yazdan-Shahmorad
doi: https://doi.org/10.1101/2021.08.04.455066
Karam Khateeb
1Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
2Washington National Primate Research Center, Seattle, WA 98195, USA
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Julien Bloch
1Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
2Washington National Primate Research Center, Seattle, WA 98195, USA
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Jasmine Zhou
1Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
2Washington National Primate Research Center, Seattle, WA 98195, USA
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Mona Rahimi
2Washington National Primate Research Center, Seattle, WA 98195, USA
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Devon J. Griggs
2Washington National Primate Research Center, Seattle, WA 98195, USA
3Department of Electrical and Computer Engineering, University of Washington, Seattle, WA 98195, USA
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Viktor N. Kharazia
4Department of Physiology and Center for Integrative Neuroscience, University of California, San Francisco, CA 94143, USA
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Min N. Le
1Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
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Ruikang Wang
1Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
5Department of Ophthalmology, University of Washington Medicine, Seattle, WA 98195, USA
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Azadeh Yazdan-Shahmorad
1Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
2Washington National Primate Research Center, Seattle, WA 98195, USA
3Department of Electrical and Computer Engineering, University of Washington, Seattle, WA 98195, USA
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  • For correspondence: azadehy@uw.edu
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Summary

Lesioning and neurophysiological studies have facilitated the elucidation of cortical functions and mechanisms of functional recovery following injury. Clinical translation of such studies is contingent on their employment in non-human primates (NHPs), yet tools for monitoring and modulating cortical physiology are incompatible with conventional NHP lesioning techniques. To address these challenges, we developed a toolbox demonstrated in seven macaques. We introduce the photothrombotic method to induce focal cortical lesions alongside a quantitative model for the design of lesion profiles based on experimental needs. Large-scale (∼5 cm2) vascular and neural dynamics can be monitored and lesion induction can be validated in vivo with optical coherence tomography angiography and our electrocorticographic array, the latter of which also enables testing stimulation-based interventions. By combining optical and electrophysiological techniques in NHPs, we can enhance our understanding of cortical functions, investigate functional recovery mechanisms, integrate physiological and behavioral findings, and develop treatments for neurological disorders.

Competing Interest Statement

RKW discloses intellectual property owned by the Oregon Health and Science University and the University of Washington. He is a consultant to Carl Zeiss Meditec. The remaining authors declare no disclosures.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted August 05, 2021.
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A toolbox for studying cortical physiology in primates
Karam Khateeb, Julien Bloch, Jasmine Zhou, Mona Rahimi, Devon J. Griggs, Viktor N. Kharazia, Min N. Le, Ruikang Wang, Azadeh Yazdan-Shahmorad
bioRxiv 2021.08.04.455066; doi: https://doi.org/10.1101/2021.08.04.455066
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A toolbox for studying cortical physiology in primates
Karam Khateeb, Julien Bloch, Jasmine Zhou, Mona Rahimi, Devon J. Griggs, Viktor N. Kharazia, Min N. Le, Ruikang Wang, Azadeh Yazdan-Shahmorad
bioRxiv 2021.08.04.455066; doi: https://doi.org/10.1101/2021.08.04.455066

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