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uPIC–M: efficient and scalable preparation of clonal single mutant libraries for high-throughput protein biochemistry

View ORCID ProfileMason J. Appel, View ORCID ProfileScott A. Longwell, Maurizio Morri, Norma Neff, View ORCID ProfileDaniel Herschlag, View ORCID ProfilePolly M. Fordyce
doi: https://doi.org/10.1101/2021.08.04.455146
Mason J. Appel
1Department of Biochemistry, Stanford University, Stanford, CA 94305
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Scott A. Longwell
2Department of Bioengineering, Stanford University, Stanford, CA 94305
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Maurizio Morri
3Chan Zuckerberg Biohub, San Francisco, CA 94110
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Norma Neff
3Chan Zuckerberg Biohub, San Francisco, CA 94110
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Daniel Herschlag
1Department of Biochemistry, Stanford University, Stanford, CA 94305
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  • For correspondence: herschla@stanford.edu pfordyce@stanford.edu
Polly M. Fordyce
2Department of Bioengineering, Stanford University, Stanford, CA 94305
3Chan Zuckerberg Biohub, San Francisco, CA 94110
4Department of Genetics, Stanford University, Stanford, CA 94305
5ChEM-H Institute, Stanford University, Stanford, CA 94305
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  • For correspondence: herschla@stanford.edu pfordyce@stanford.edu
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Article Information

doi 
https://doi.org/10.1101/2021.08.04.455146
History 
  • August 4, 2021.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. Mason J. Appel1,
  2. Scott A. Longwell2,
  3. Maurizio Morri3,
  4. Norma Neff3,
  5. Daniel Herschlag1,‡ and
  6. Polly M. Fordyce2,3,4,5,‡
  1. 1Department of Biochemistry, Stanford University, Stanford, CA 94305
  2. 2Department of Bioengineering, Stanford University, Stanford, CA 94305
  3. 3Chan Zuckerberg Biohub, San Francisco, CA 94110
  4. 4Department of Genetics, Stanford University, Stanford, CA 94305
  5. 5ChEM-H Institute, Stanford University, Stanford, CA 94305
  1. ↵‡Corresponding authors (herschla{at}stanford.edu and pfordyce{at}stanford.edu)
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Posted August 04, 2021.
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uPIC–M: efficient and scalable preparation of clonal single mutant libraries for high-throughput protein biochemistry
Mason J. Appel, Scott A. Longwell, Maurizio Morri, Norma Neff, Daniel Herschlag, Polly M. Fordyce
bioRxiv 2021.08.04.455146; doi: https://doi.org/10.1101/2021.08.04.455146
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uPIC–M: efficient and scalable preparation of clonal single mutant libraries for high-throughput protein biochemistry
Mason J. Appel, Scott A. Longwell, Maurizio Morri, Norma Neff, Daniel Herschlag, Polly M. Fordyce
bioRxiv 2021.08.04.455146; doi: https://doi.org/10.1101/2021.08.04.455146

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