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Large field-of-view incoherent volumetric imaging in living human retina by confocal oblique scanning laser ophthalmoscopy

Wenjun Shao, Ji Yi
doi: https://doi.org/10.1101/2021.08.05.455286
Wenjun Shao
1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, 21231, USA
2Department of Ophthalmology, Johns Hopkins University, Baltimore, Maryland, 21231, USA
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Ji Yi
1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, 21231, USA
2Department of Ophthalmology, Johns Hopkins University, Baltimore, Maryland, 21231, USA
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  • For correspondence: jiyi@jhu.edu
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Abstract

Three-dimensional (3D) volumetric imaging of the human retina is instrumental to monitor and diagnose blinding conditions. Although coherent retinal imaging is well established by optical coherence tomography, it is still a large void for incoherent volumetric imaging in the human retina. Here, we report confocal oblique scanning laser ophthalmoscopy (CoSLO), to fill that void and harness incoherent optical contrast in 3D. CoSLO uses oblique scanning laser and remote focusing to acquire depth signal in parallel, avoid the lengthy z-stacking, and image a large field of view (FOV). In addition, confocal gating is introduced by a linear sensor array to improve the contrast and resolution. For the first time, we achieved incoherent 3D human retinal imaging with >20° viewing angle within only 5 seconds. The depth resolution is ∼45 microns in vivo. We demonstrated label-free incoherent contrast by CoSLO, revealing unique features in the retina. CoSLO will be an important technique for clinical care of retinal conditions and fundamental vision science, by offering unique volumetric incoherent contrasts.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • There was a typo in the previous version. The name of the author is changed from Yi Ji to Ji Yi.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 07, 2021.
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Large field-of-view incoherent volumetric imaging in living human retina by confocal oblique scanning laser ophthalmoscopy
Wenjun Shao, Ji Yi
bioRxiv 2021.08.05.455286; doi: https://doi.org/10.1101/2021.08.05.455286
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Large field-of-view incoherent volumetric imaging in living human retina by confocal oblique scanning laser ophthalmoscopy
Wenjun Shao, Ji Yi
bioRxiv 2021.08.05.455286; doi: https://doi.org/10.1101/2021.08.05.455286

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