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OPAM, an Open source, 3D printed Low-cost Micro-Manipulator for Single Cell Manipulation

View ORCID ProfileJiang Xu, Zhuowei Du, Paul Liu, Yi Kou, Lin Chen
doi: https://doi.org/10.1101/2021.08.09.455588
Jiang Xu
1Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, United States
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  • ORCID record for Jiang Xu
  • For correspondence: foxjuly@gmail.com linchen@usc.edu
Zhuowei Du
1Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, United States
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Paul Liu
1Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, United States
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Yi Kou
1Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, United States
2Department of Chemistry, University of Southern California, Los Angeles, CA, 90089, United States
3USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA
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Lin Chen
1Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, United States
2Department of Chemistry, University of Southern California, Los Angeles, CA, 90089, United States
3USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA
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  • For correspondence: foxjuly@gmail.com linchen@usc.edu
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Abstract

We introduce OPAM, an Open source, low-cost (under $150), 3D-Printed, stepper motor driven, Arduino based, single cell Micromanipulator (OPAM). Modification of a commercial stepper motor led to dramatically increased stability and maneuverability of the motor, based on which the micromanipulator was designed. All components of this micromanipulator can be 3D printed using an entry-level 3D printer and assembled with ease. With this single cell manipulator, successful targeted single cell capture and transfer was confirmed under the microscope, which showed great promise for single cell related experiments.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://github.com/JiangXu123/DIY_Lab_Instrument/tree/master/micro_manipulator

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted August 10, 2021.
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OPAM, an Open source, 3D printed Low-cost Micro-Manipulator for Single Cell Manipulation
Jiang Xu, Zhuowei Du, Paul Liu, Yi Kou, Lin Chen
bioRxiv 2021.08.09.455588; doi: https://doi.org/10.1101/2021.08.09.455588
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OPAM, an Open source, 3D printed Low-cost Micro-Manipulator for Single Cell Manipulation
Jiang Xu, Zhuowei Du, Paul Liu, Yi Kou, Lin Chen
bioRxiv 2021.08.09.455588; doi: https://doi.org/10.1101/2021.08.09.455588

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