Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Single-molecule simultaneous profiling of DNA methylation and DNA-protein interactions with Nanopore-DamID

View ORCID ProfileSeth W. Cheetham, Yohaann M. A. Jafrani, Stacey B. Andersen, Natasha Jansz, Adam D. Ewing, Geoffrey J. Faulkner
doi: https://doi.org/10.1101/2021.08.09.455753
Seth W. Cheetham
1Mater Research Institute – University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Seth W. Cheetham
  • For correspondence: seth.cheetham@mater.uq.edu.au
Yohaann M. A. Jafrani
1Mater Research Institute – University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stacey B. Andersen
2Genome Innovation Hub, The University of Queensland, Brisbane, QLD 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Natasha Jansz
1Mater Research Institute – University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Adam D. Ewing
1Mater Research Institute – University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Geoffrey J. Faulkner
1Mater Research Institute – University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia
3Queensland Brain Institute, University of Queensland, Brisbane QLD 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: seth.cheetham@mater.uq.edu.au
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

DNA-protein interactions and cytosine methylation control eukaryotic gene expression. Here, we present an approach to simultaneously detect cytosine methylation and DNA-protein interactions from single molecules, through selective sequencing of adenine-labelled DNA. Applying this approach to LaminB1-associated heterochromatin domains, we identify strict CpG methylation maintenance at transcriptional start sites amidst a generalised relaxation of methylation, potentially to prevent ectopic and aberrant heterochromatic gene expression.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted August 10, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Single-molecule simultaneous profiling of DNA methylation and DNA-protein interactions with Nanopore-DamID
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Single-molecule simultaneous profiling of DNA methylation and DNA-protein interactions with Nanopore-DamID
Seth W. Cheetham, Yohaann M. A. Jafrani, Stacey B. Andersen, Natasha Jansz, Adam D. Ewing, Geoffrey J. Faulkner
bioRxiv 2021.08.09.455753; doi: https://doi.org/10.1101/2021.08.09.455753
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Single-molecule simultaneous profiling of DNA methylation and DNA-protein interactions with Nanopore-DamID
Seth W. Cheetham, Yohaann M. A. Jafrani, Stacey B. Andersen, Natasha Jansz, Adam D. Ewing, Geoffrey J. Faulkner
bioRxiv 2021.08.09.455753; doi: https://doi.org/10.1101/2021.08.09.455753

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4855)
  • Biochemistry (10798)
  • Bioengineering (8045)
  • Bioinformatics (27310)
  • Biophysics (13987)
  • Cancer Biology (11127)
  • Cell Biology (16062)
  • Clinical Trials (138)
  • Developmental Biology (8788)
  • Ecology (13294)
  • Epidemiology (2067)
  • Evolutionary Biology (17364)
  • Genetics (11689)
  • Genomics (15925)
  • Immunology (11034)
  • Microbiology (26093)
  • Molecular Biology (10654)
  • Neuroscience (56570)
  • Paleontology (418)
  • Pathology (1732)
  • Pharmacology and Toxicology (3005)
  • Physiology (4547)
  • Plant Biology (9630)
  • Scientific Communication and Education (1615)
  • Synthetic Biology (2689)
  • Systems Biology (6979)
  • Zoology (1510)