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Long-term Transverse Imaging of the Hippocampus with Glass Microperiscopes

William T Redman, Nora S Wolcott, Luca Montelisciani, Gabriel Luna, Tyler D Marks, Kevin K Sit, Che-Hang Yu, Spencer L Smith, View ORCID ProfileMichael J Goard
doi: https://doi.org/10.1101/2021.08.10.455885
William T Redman
1Interdepartmental Graduate Program in Dynamical Neuroscience, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Nora S Wolcott
2Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Luca Montelisciani
3Cognitive and Systems Neuroscience Group, University of Amsterdam, Amsterdam, NL 1098 XH
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Gabriel Luna
4Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Tyler D Marks
4Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Kevin K Sit
5Department of Psychological and Brain Sciences, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Che-Hang Yu
6Department of Electrical and Computer Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Spencer L Smith
4Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
6Department of Electrical and Computer Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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Michael J Goard
2Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
4Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
5Department of Psychological and Brain Sciences, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
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  • ORCID record for Michael J Goard
  • For correspondence: michael.goard@lifesci.ucsb.edu
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ABSTRACT

The hippocampus consists of a stereotyped neuronal circuit repeated along the septal-temporal axis. This transverse circuit contains distinct subfields with stereotyped connectivity that support crucial cognitive processes, including episodic and spatial memory. However, comprehensive measurements across the transverse hippocampal circuit in vivo are intractable with existing techniques. Here, we developed an approach for two-photon imaging of the transverse hippocampal plane in awake mice via implanted glass microperiscopes, allowing optical access to the major hippocampal subfields and to the dendritic arbor of pyramidal neurons. Using this approach, we tracked dendritic morphological dynamics on CA1 apical dendrites and characterized spine turnover. We then used calcium imaging to quantify the prevalence of place and speed cells across subfields. Finally, we measured the anatomical distribution of spatial information, finding a non-uniform distribution of spatial selectivity along the DG-to-CA1 axis. This approach extends the existing toolbox for structural and functional measurements of hippocampal circuitry.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted August 11, 2021.
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Long-term Transverse Imaging of the Hippocampus with Glass Microperiscopes
William T Redman, Nora S Wolcott, Luca Montelisciani, Gabriel Luna, Tyler D Marks, Kevin K Sit, Che-Hang Yu, Spencer L Smith, Michael J Goard
bioRxiv 2021.08.10.455885; doi: https://doi.org/10.1101/2021.08.10.455885
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Long-term Transverse Imaging of the Hippocampus with Glass Microperiscopes
William T Redman, Nora S Wolcott, Luca Montelisciani, Gabriel Luna, Tyler D Marks, Kevin K Sit, Che-Hang Yu, Spencer L Smith, Michael J Goard
bioRxiv 2021.08.10.455885; doi: https://doi.org/10.1101/2021.08.10.455885

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