Abstract
Fluorescence lifetime imaging (FLIM) allows the quantification of subcellular processes in situ, in living cells. A number of approaches have been developed to extract the lifetime from time-domain FLIM data, but they are often limited in terms of dynamic range, speed, photon efficiency or precision. Here, we focus on one of the best performing methods in the field, the center-of-mass (CMM) method, that conveys advantages in terms of speed and photon efficiency over others. In this paper, however, we identify a loss of photon efficiency of CMM for short lifetimes when background noise is present. We sub-sequently present a new development and generalization of the CMM method that provides for the rapid and accurate extraction of fluorescence lifetime over a large lifetime dynamic range. We provide software tools to simulate, validate and analyze FLIM data sets and compare the performance of our approach against the standard CMM and the commonly employed leastsquare minimization (LSM) methods. Our method features a better photon efficiency than standard CMM and LSM and is robust in the presence of background noise. The algorithm is applicable to any time-domain FLIM dataset.
Competing Interest Statement
The authors have declared no competing interest.