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Reconstructing transcriptional histories by CRISPR acquisition of retron-based genetic barcodes

View ORCID ProfileSanti Bhattarai-Kline, View ORCID ProfileElana Lockshin, View ORCID ProfileMax G. Schubert, View ORCID ProfileJeff Nivala, View ORCID ProfileGeorge Church, View ORCID ProfileSeth L. Shipman
doi: https://doi.org/10.1101/2021.08.11.455990
Santi Bhattarai-Kline
1Gladstone Institute of Data Science and Biotechnology, San Francisco, CA, USA
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  • ORCID record for Santi Bhattarai-Kline
Elana Lockshin
2Department of Neurobiology, Duke University Medical Center, Durham, NC, USA
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  • ORCID record for Elana Lockshin
Max G. Schubert
3Department of Genetics, Harvard Medical School, Boston, MA, USA
4Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA
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Jeff Nivala
5Paul G. Allen School of Computer Science & Engineering, University of Washington, Seattle, WA, USA
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  • ORCID record for Jeff Nivala
George Church
3Department of Genetics, Harvard Medical School, Boston, MA, USA
4Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA
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Seth L. Shipman
1Gladstone Institute of Data Science and Biotechnology, San Francisco, CA, USA
6Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, USA
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  • For correspondence: seth.shipman@gladstone.ucsf.edu
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Article Information

doi 
https://doi.org/10.1101/2021.08.11.455990
History 
  • August 12, 2021.

Article Versions

  • Version 1 (August 11, 2021 - 19:43).
  • You are currently viewing Version 2 of this article (August 12, 2021 - 20:34).
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Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. Santi Bhattarai-Kline1,
  2. Elana Lockshin2,
  3. Max G. Schubert3,4,
  4. Jeff Nivala5,
  5. George Church3,4 and
  6. Seth L. Shipman1,6,*
  1. 1Gladstone Institute of Data Science and Biotechnology, San Francisco, CA, USA
  2. 2Department of Neurobiology, Duke University Medical Center, Durham, NC, USA
  3. 3Department of Genetics, Harvard Medical School, Boston, MA, USA
  4. 4Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA
  5. 5Paul G. Allen School of Computer Science & Engineering, University of Washington, Seattle, WA, USA
  6. 6Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, USA
  1. ↵*CORRESPONDING AUTHOR
    Correspondence to Seth L. Shipman, Correspondence to: seth.shipman{at}gladstone.ucsf.edu
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Posted August 12, 2021.
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Reconstructing transcriptional histories by CRISPR acquisition of retron-based genetic barcodes
Santi Bhattarai-Kline, Elana Lockshin, Max G. Schubert, Jeff Nivala, George Church, Seth L. Shipman
bioRxiv 2021.08.11.455990; doi: https://doi.org/10.1101/2021.08.11.455990
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Reconstructing transcriptional histories by CRISPR acquisition of retron-based genetic barcodes
Santi Bhattarai-Kline, Elana Lockshin, Max G. Schubert, Jeff Nivala, George Church, Seth L. Shipman
bioRxiv 2021.08.11.455990; doi: https://doi.org/10.1101/2021.08.11.455990

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