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Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

View ORCID ProfileJoël S. Bloch, View ORCID ProfileSomnath Mukherjee, View ORCID ProfileJulia Kowal, View ORCID ProfileEkaterina V. Filippova, Martina Niederer, View ORCID ProfileEls Pardon, View ORCID ProfileJan Steyaert, View ORCID ProfileAnthony A. Kossiakoff, View ORCID ProfileKaspar P. Locher
doi: https://doi.org/10.1101/2021.08.20.457137
Joël S. Bloch
1Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland
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  • ORCID record for Joël S. Bloch
Somnath Mukherjee
2Department of Biochemistry and Molecular Biology, University of Chicago, 900 East 57th Street, Chicago, IL 60637, USA
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Julia Kowal
1Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland
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Ekaterina V. Filippova
2Department of Biochemistry and Molecular Biology, University of Chicago, 900 East 57th Street, Chicago, IL 60637, USA
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  • ORCID record for Ekaterina V. Filippova
Martina Niederer
1Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland
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Els Pardon
4Structural Biology Brussels, Vrije Universiteit Brussel, VUB, Brussels, Belgium
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Jan Steyaert
4Structural Biology Brussels, Vrije Universiteit Brussel, VUB, Brussels, Belgium
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Anthony A. Kossiakoff
2Department of Biochemistry and Molecular Biology, University of Chicago, 900 East 57th Street, Chicago, IL 60637, USA
3Institute for Biophysical Dynamics, University of Chicago, Chicago, IL, USA
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  • For correspondence: locher@mol.biol.ethz.ch koss@bsd.uchicago.edu
Kaspar P. Locher
1Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland
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  • ORCID record for Kaspar P. Locher
  • For correspondence: locher@mol.biol.ethz.ch koss@bsd.uchicago.edu
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Article Information

doi 
https://doi.org/10.1101/2021.08.20.457137
History 
  • August 20, 2021.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Joël S. Bloch1,5,
  2. Somnath Mukherjee2,5,
  3. Julia Kowal1,
  4. Ekaterina V. Filippova2,
  5. Martina Niederer1,
  6. Els Pardon4,
  7. Jan Steyaert4,
  8. Anthony A. Kossiakoff2,3,* and
  9. Kaspar P. Locher1,*
  1. 1Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland
  2. 2Department of Biochemistry and Molecular Biology, University of Chicago, 900 East 57th Street, Chicago, IL 60637, USA
  3. 3Institute for Biophysical Dynamics, University of Chicago, Chicago, IL, USA
  4. 4Structural Biology Brussels, Vrije Universiteit Brussel, VUB, Brussels, Belgium
  1. ↵*To whom correspondence should be addressed: locher{at}mol.biol.ethz.ch, koss{at}bsd.uchicago.edu
  1. ↵5 These authors contributed equally

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Posted August 20, 2021.
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Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins
Joël S. Bloch, Somnath Mukherjee, Julia Kowal, Ekaterina V. Filippova, Martina Niederer, Els Pardon, Jan Steyaert, Anthony A. Kossiakoff, Kaspar P. Locher
bioRxiv 2021.08.20.457137; doi: https://doi.org/10.1101/2021.08.20.457137
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Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins
Joël S. Bloch, Somnath Mukherjee, Julia Kowal, Ekaterina V. Filippova, Martina Niederer, Els Pardon, Jan Steyaert, Anthony A. Kossiakoff, Kaspar P. Locher
bioRxiv 2021.08.20.457137; doi: https://doi.org/10.1101/2021.08.20.457137

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