Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

Joël S. Bloch; Somnath Mukherjee; Julia Kowal; Ekaterina V. Filippova; Martina Niederer; Els Pardon; Jan Steyaert; Anthony A. Kossiakoff; Kaspar P. Locher

doi:10.1101/2021.08.20.457137

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Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

View ORCID ProfileJoël S. Bloch, View ORCID ProfileSomnath Mukherjee, View ORCID ProfileJulia Kowal, View ORCID ProfileEkaterina V. Filippova, Martina Niederer, View ORCID ProfileEls Pardon, View ORCID ProfileJan Steyaert, View ORCID ProfileAnthony A. Kossiakoff, View ORCID ProfileKaspar P. Locher

doi: https://doi.org/10.1101/2021.08.20.457137

Joël S. Bloch

1Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland

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Somnath Mukherjee

2Department of Biochemistry and Molecular Biology, University of Chicago, 900 East 57th Street, Chicago, IL 60637, USA

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Julia Kowal

1Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland

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Ekaterina V. Filippova

2Department of Biochemistry and Molecular Biology, University of Chicago, 900 East 57th Street, Chicago, IL 60637, USA

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ORCID record for Ekaterina V. Filippova

Martina Niederer

1Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland

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Els Pardon

4Structural Biology Brussels, Vrije Universiteit Brussel, VUB, Brussels, Belgium

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Jan Steyaert

4Structural Biology Brussels, Vrije Universiteit Brussel, VUB, Brussels, Belgium

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Anthony A. Kossiakoff

2Department of Biochemistry and Molecular Biology, University of Chicago, 900 East 57th Street, Chicago, IL 60637, USA

3Institute for Biophysical Dynamics, University of Chicago, Chicago, IL, USA

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For correspondence: locher@mol.biol.ethz.ch koss@bsd.uchicago.edu

Kaspar P. Locher

1Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland

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For correspondence: locher@mol.biol.ethz.ch koss@bsd.uchicago.edu

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Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

Joël S. Bloch, Somnath Mukherjee, Julia Kowal, Ekaterina V. Filippova, Martina Niederer, Els Pardon, Jan Steyaert, Anthony A. Kossiakoff, Kaspar P. Locher

bioRxiv 2021.08.20.457137; doi: https://doi.org/10.1101/2021.08.20.457137

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Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

Joël S. Bloch, Somnath Mukherjee, Julia Kowal, Ekaterina V. Filippova, Martina Niederer, Els Pardon, Jan Steyaert, Anthony A. Kossiakoff, Kaspar P. Locher

bioRxiv 2021.08.20.457137; doi: https://doi.org/10.1101/2021.08.20.457137

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