Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Acquisition of cell identity in the brown alga Ectocarpus: which of time, cell shape or position matters most?

View ORCID ProfileBernard Billoud, View ORCID ProfileDenis Saint-Marcoux, Sabine Chenivesse, Carole Duchêne, Camille Noûs, View ORCID ProfileJane A. Langdale, View ORCID ProfileBénédicte Charrier
doi: https://doi.org/10.1101/2021.08.21.457218
Bernard Billoud
1Laboratoire de Biologie Intégrative des Modèles Marins, UMR8227 CNRS-Sorbonne Université, Station Biologique, Place Georges Teissier, 29280 Roscoff, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Bernard Billoud
Denis Saint-Marcoux
2Department of Plant Sciences, University of Oxford, South Parks Rd., Oxford OX1 3RB, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Denis Saint-Marcoux
Sabine Chenivesse
1Laboratoire de Biologie Intégrative des Modèles Marins, UMR8227 CNRS-Sorbonne Université, Station Biologique, Place Georges Teissier, 29280 Roscoff, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carole Duchêne
1Laboratoire de Biologie Intégrative des Modèles Marins, UMR8227 CNRS-Sorbonne Université, Station Biologique, Place Georges Teissier, 29280 Roscoff, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Camille Noûs
3Laboratoire Cogitamus
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jane A. Langdale
2Department of Plant Sciences, University of Oxford, South Parks Rd., Oxford OX1 3RB, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jane A. Langdale
Bénédicte Charrier
1Laboratoire de Biologie Intégrative des Modèles Marins, UMR8227 CNRS-Sorbonne Université, Station Biologique, Place Georges Teissier, 29280 Roscoff, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Bénédicte Charrier
  • For correspondence: benedicte.charrier@sb-roscoff.fr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

During development, cells undergo simultaneous changes of different types that together depict cell “identity”. In the multicellular brown alga Ectocarpus sp., while ageing, cells change shape and relative position within the filament. Understanding how these factors act and interact to specify cell identity requires markers of cell identity and the ability to genetically separate age, shape and position. Here we used laser capture microdissection (LCM) to isolate specific cell types from young sporophytes of Ectocarpus, and performed differential RNA-seq analysis. Transcriptome profiles of cell types in the wild-type strain provided signatures of the five cell types that can be identified by shape and position. In two mutants, where the relationship between cell shape, position and age are altered, transcriptome signatures revealed that little differential expression could be identified when only shape was perturbed. More generally, although the two mutants are characterised by opposite morphological phenotypes, their transcriptomes were remarkably similar. We concluded that despite the robustness of cell differentiation during WT development, neither the shape nor the position of the cell could serve as a faithful gauge for tracking differentiation.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted August 22, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Acquisition of cell identity in the brown alga Ectocarpus: which of time, cell shape or position matters most?
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Acquisition of cell identity in the brown alga Ectocarpus: which of time, cell shape or position matters most?
Bernard Billoud, Denis Saint-Marcoux, Sabine Chenivesse, Carole Duchêne, Camille Noûs, Jane A. Langdale, Bénédicte Charrier
bioRxiv 2021.08.21.457218; doi: https://doi.org/10.1101/2021.08.21.457218
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Acquisition of cell identity in the brown alga Ectocarpus: which of time, cell shape or position matters most?
Bernard Billoud, Denis Saint-Marcoux, Sabine Chenivesse, Carole Duchêne, Camille Noûs, Jane A. Langdale, Bénédicte Charrier
bioRxiv 2021.08.21.457218; doi: https://doi.org/10.1101/2021.08.21.457218

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4687)
  • Biochemistry (10371)
  • Bioengineering (7689)
  • Bioinformatics (26358)
  • Biophysics (13544)
  • Cancer Biology (10713)
  • Cell Biology (15452)
  • Clinical Trials (138)
  • Developmental Biology (8508)
  • Ecology (12831)
  • Epidemiology (2067)
  • Evolutionary Biology (16877)
  • Genetics (11407)
  • Genomics (15489)
  • Immunology (10632)
  • Microbiology (25242)
  • Molecular Biology (10233)
  • Neuroscience (54566)
  • Paleontology (402)
  • Pathology (1670)
  • Pharmacology and Toxicology (2899)
  • Physiology (4350)
  • Plant Biology (9263)
  • Scientific Communication and Education (1587)
  • Synthetic Biology (2558)
  • Systems Biology (6786)
  • Zoology (1470)