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Tracheal tube fusion in Drosophila involves release of extracellular vesicles from multivesicular bodies

Carolina Camelo, Anna Körte, Thea Jacobs, View ORCID ProfileStefan Luschnig
doi: https://doi.org/10.1101/2021.08.22.457102
Carolina Camelo
1Institute of Animal Physiology, University of Münster, D-48143 Münster, Germany
2Cells in Motion (CiM) Interfaculty Centre, D-48149 Münster, Germany
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Anna Körte
1Institute of Animal Physiology, University of Münster, D-48143 Münster, Germany
2Cells in Motion (CiM) Interfaculty Centre, D-48149 Münster, Germany
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Thea Jacobs
1Institute of Animal Physiology, University of Münster, D-48143 Münster, Germany
2Cells in Motion (CiM) Interfaculty Centre, D-48149 Münster, Germany
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Stefan Luschnig
1Institute of Animal Physiology, University of Münster, D-48143 Münster, Germany
2Cells in Motion (CiM) Interfaculty Centre, D-48149 Münster, Germany
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  • ORCID record for Stefan Luschnig
  • For correspondence: luschnig@uni-muenster.de
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Summary

Extracellular vesicles (EVs) comprise diverse types of cell-released membranous structures that are thought to play important roles in intercellular communication. Despite extensive work on the formation and functions of EVs in cultured cells, studies of EVs in vivo have remained scarce. We report here that EVs are present in the developing lumen of tracheal tubes in Drosophila embryos. We defined two distinct EV subpopulations, one of which contains the Munc13-4 homologue Staccato (Stac) and is spatially and temporally associated with tracheal tube fusion events. The formation of Stac-positive luminal EVs depends on the tip-cell-specific GTPase Arl3, which is also required for the formation of Stac-positive multivesicular bodies, suggesting that Stac-EVs derive from fusion of Stac-MVBs with the luminal membrane in tip cells during anastomosis formation. The GTPases Rab27 and Rab35 cooperate downstream of Arl3 to promote Stac-MVB formation and tube fusion. We propose that Stac-MVBs act as membrane reservoirs that facilitate tracheal lumen fusion in a process regulated by Arl3, Rab27, Rab35, and Stac/Munc13-4.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • This version of the manuscript has been revised to include changes to the abstract and main text and to Figure 1 and Figure S5. One of the authors on the original preprint, Dr. Peter Robin Hiesinger, refrained from authorship on the revised manuscript, as his contribution to the work (provisioning of the collection of Rab null mutants) got published independently in the meantime.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted November 02, 2021.
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Tracheal tube fusion in Drosophila involves release of extracellular vesicles from multivesicular bodies
Carolina Camelo, Anna Körte, Thea Jacobs, Stefan Luschnig
bioRxiv 2021.08.22.457102; doi: https://doi.org/10.1101/2021.08.22.457102
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Tracheal tube fusion in Drosophila involves release of extracellular vesicles from multivesicular bodies
Carolina Camelo, Anna Körte, Thea Jacobs, Stefan Luschnig
bioRxiv 2021.08.22.457102; doi: https://doi.org/10.1101/2021.08.22.457102

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