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Kif9 is an active kinesin motor required for ciliary beating and proximodistal patterning of motile axonemes

Mia J. Konjikusic, Chanjae Lee, Yang Yue, Bikram D. Shrestha, Ange M. Nguimtsop, Amjad Horani, Steven Brody, Vivek N. Prakash, Ryan S. Gray, Kristen J. Verhey, John B. Wallingford
doi: https://doi.org/10.1101/2021.08.26.457815
Mia J. Konjikusic
1Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA
2Department of Pediatrics, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd, The University of Texas at Austin, Dell Medical School, Austin, TX, USA
3th, Austin, TX 78712, USA
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Chanjae Lee
1Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA
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Yang Yue
4Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA
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Bikram D. Shrestha
5Department of Physics, University of Miami, Coral Gables, FL, USA
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Ange M. Nguimtsop
1Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA
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Amjad Horani
6Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA
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Steven Brody
7Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA
8Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, 63110 USA
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Vivek N. Prakash
5Department of Physics, University of Miami, Coral Gables, FL, USA
9Department of Biology and Department of Marine Biology and Ecology, University of Miami, Coral Gables, FL, USA
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Ryan S. Gray
2Department of Pediatrics, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd, The University of Texas at Austin, Dell Medical School, Austin, TX, USA
3th, Austin, TX 78712, USA
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Kristen J. Verhey
4Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA
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John B. Wallingford
1Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA
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  • For correspondence: Wallingford@austin.utexas.edu
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Abstract

Most motile cilia have a stereotyped structure of nine microtubule outer doublets and a single central pair of microtubules. The central pair microtubules are surrounded by a set of proteins, termed the central pair apparatus. A specific kinesin, Klp1 projects from the central pair and contributes to ciliary motility in Chlamydomonas. The vertebrate orthologue, Kif9 is required for beating in mouse sperm flagella, but the mechanism of Kif9/Klp1 function remains poorly defined. Here, using Xenopus epidermal multiciliated cells, we show that Kif9 is necessary for ciliary motility as well as leads to defects in the distal localization of not only central pair proteins, but also radial spokes and dynein arms. In addition, single-molecule assays in vitro revealed that Xenopus Kif9 is a processive motor, though like axonemal dyneins it displays no processivity in ciliary axonemes in vivo. Thus, our data suggest that Kif9 plays both indirect and direct role in ciliary motility.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted August 27, 2021.
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Kif9 is an active kinesin motor required for ciliary beating and proximodistal patterning of motile axonemes
Mia J. Konjikusic, Chanjae Lee, Yang Yue, Bikram D. Shrestha, Ange M. Nguimtsop, Amjad Horani, Steven Brody, Vivek N. Prakash, Ryan S. Gray, Kristen J. Verhey, John B. Wallingford
bioRxiv 2021.08.26.457815; doi: https://doi.org/10.1101/2021.08.26.457815
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Kif9 is an active kinesin motor required for ciliary beating and proximodistal patterning of motile axonemes
Mia J. Konjikusic, Chanjae Lee, Yang Yue, Bikram D. Shrestha, Ange M. Nguimtsop, Amjad Horani, Steven Brody, Vivek N. Prakash, Ryan S. Gray, Kristen J. Verhey, John B. Wallingford
bioRxiv 2021.08.26.457815; doi: https://doi.org/10.1101/2021.08.26.457815

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