Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

β-glucan imprinting remodels macrophage function in response to environmental cues

View ORCID ProfileAlícia C. Piffer, View ORCID ProfileGiorgio Camilli, View ORCID ProfileMathieu Bohm, Rachel Lavenir, View ORCID ProfileJessica Quintin
doi: https://doi.org/10.1101/2021.08.30.458241
Alícia C. Piffer
*Institut Pasteur, Université de Paris, Immunology of Fungal Infections – F-75015 Paris, France
†Laboratório de Glicobiologia de Eucariotos, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro – Brasil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alícia C. Piffer
Giorgio Camilli
*Institut Pasteur, Université de Paris, Immunology of Fungal Infections – F-75015 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Giorgio Camilli
Mathieu Bohm
*Institut Pasteur, Université de Paris, Immunology of Fungal Infections – F-75015 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mathieu Bohm
Rachel Lavenir
*Institut Pasteur, Université de Paris, Immunology of Fungal Infections – F-75015 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jessica Quintin
*Institut Pasteur, Université de Paris, Immunology of Fungal Infections – F-75015 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jessica Quintin
  • For correspondence: jessica.quintin@pasteur.fr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

In vitro, exposure of human primary monocytes to the fungal β-glucan enhances their pro-inflammatory responsiveness towards several pathogens. Yet, the role of environmental condition of this process remains unclear. Here we found that β-glucan-induced innate immune memory counteract the anti-inflammatory status of the macrophages. In response to β-glucan imprinting, M-CSF-(M2-like-) macrophages increase their pro-inflammatory responsiveness to secondary stimuli associated with decrease of the M-CSF differentiation hallmarks. In contrast, in GM-CSF-(M1-like-) environment, β-glucan imprinting reduced the pro-inflammatory canonical feature of the macrophages, together with their terminal differentiation marks. Comparing M-CSF and GM-CSF environment, we observed that β-glucan-imprinted macrophages present comparable functions in terms of cytokine responses, phagocytosis, oxidative burst, and angiogenesis. This effect is mediated through Dectin-1 and associated with altered expression of the master regulators of macrophage terminal differentiation, IRF5 and IRF3. β-glucan-induced innate immune memory skews the commitment of the macrophages in complex environment towards similar and less terminally differentiated cells. Together, these observations suggest a potential therapeutic role for β-glucan-induced modulation of innate memory in different pathological contexts.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • 1 A.C.P, G.C., M.B., R.L., and J.Q. were supported by the ANR JCJC grant (ANR-16-CE15-0014-01), and by Institut Carnot–Microbes et Santé grant (n°11 CARN-017-01) (to J.Q.). The authors also acknowledge funding from CAPES finance code 001. A.C.P. was supported by the CAPES-COFECUB Franco-Brazilian Research Exchange Program (88887.281641/2018-00). For the purpose of open access, the author has applied a CC BY public copyright license to any author accepted manuscript version arising from this submission.

  • This revision is a full-length version of the article.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted November 06, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
β-glucan imprinting remodels macrophage function in response to environmental cues
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
β-glucan imprinting remodels macrophage function in response to environmental cues
Alícia C. Piffer, Giorgio Camilli, Mathieu Bohm, Rachel Lavenir, Jessica Quintin
bioRxiv 2021.08.30.458241; doi: https://doi.org/10.1101/2021.08.30.458241
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
β-glucan imprinting remodels macrophage function in response to environmental cues
Alícia C. Piffer, Giorgio Camilli, Mathieu Bohm, Rachel Lavenir, Jessica Quintin
bioRxiv 2021.08.30.458241; doi: https://doi.org/10.1101/2021.08.30.458241

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Immunology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4654)
  • Biochemistry (10298)
  • Bioengineering (7614)
  • Bioinformatics (26189)
  • Biophysics (13445)
  • Cancer Biology (10620)
  • Cell Biology (15333)
  • Clinical Trials (138)
  • Developmental Biology (8452)
  • Ecology (12753)
  • Epidemiology (2067)
  • Evolutionary Biology (16760)
  • Genetics (11356)
  • Genomics (15399)
  • Immunology (10548)
  • Microbiology (25040)
  • Molecular Biology (10151)
  • Neuroscience (54086)
  • Paleontology (398)
  • Pathology (1655)
  • Pharmacology and Toxicology (2877)
  • Physiology (4314)
  • Plant Biology (9196)
  • Scientific Communication and Education (1579)
  • Synthetic Biology (2541)
  • Systems Biology (6752)
  • Zoology (1452)