Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Transplantation of bacteriophages from ulcerative colitis patients shifts the gut bacteriome and exacerbates severity of DSS-colitis

View ORCID ProfileAnshul Sinha, Yue Li, View ORCID ProfileMohammadali Khan Mirzaei, View ORCID ProfileMichael Shamash, Rana Samadfam, Irah L. King, View ORCID ProfileCorinne F. Maurice
doi: https://doi.org/10.1101/2021.09.10.459444
Anshul Sinha
1Department of Microbiology & Immunology, McGill University, Montreal, Quebec, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Anshul Sinha
Yue Li
1Department of Microbiology & Immunology, McGill University, Montreal, Quebec, Canada
2Department of Endocrinology and Metabolism, Guangdong Provincial Key Laboratory of Diabetology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, 510630, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mohammadali Khan Mirzaei
1Department of Microbiology & Immunology, McGill University, Montreal, Quebec, Canada
3Institute of Virology, Helmholtz Center Munich and Technical University of Munich, Neuherberg, Bavaria, 85764, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mohammadali Khan Mirzaei
Michael Shamash
1Department of Microbiology & Immunology, McGill University, Montreal, Quebec, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Michael Shamash
Rana Samadfam
4Charles River Laboratories, 22022 Transcanadienne, Senneville, QC H9X 3R3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Irah L. King
1Department of Microbiology & Immunology, McGill University, Montreal, Quebec, Canada
5McGill Interdisciplinary Initiative in Infection and Immunity, Montreal, Quebec, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: corinne.maurice@mcgill.ca irah.king@mcgill.ca
Corinne F. Maurice
1Department of Microbiology & Immunology, McGill University, Montreal, Quebec, Canada
5McGill Interdisciplinary Initiative in Infection and Immunity, Montreal, Quebec, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Corinne F. Maurice
  • For correspondence: corinne.maurice@mcgill.ca irah.king@mcgill.ca
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Data/Code
  • Preview PDF
Loading

ABSTRACT

Inflammatory bowel diseases (IBDs) including Crohn’s disease (CD) and ulcerative colitis (UC) are characterized by chronic and debilitating gut inflammation. Altered bacterial communities of the intestine are strongly associated with IBD initiation and progression. The gut virome, which is primarily composed of bacterial viruses (bacteriophages, phages) is thought to be an important factor regulating and shaping microbial communities in the gut. While alterations in the gut virome have been observed in IBD patients, the contribution of these viruses to alterations in the bacterial community and heightened inflammatory responses associated with IBD patients remains largely unknown. Here, we performed in vivo microbial cross-infection experiments to follow the effects of fecal virus-like particles (VLPs) isolated from UC patients and healthy controls on bacterial diversity and severity of experimental colitis in human microbiota-associated (HMA) mice. Shotgun metagenomics confirmed that several phages were transferred to HMA mice, resulting in treatment-specific alterations in the gut virome. VLPs from healthy and UC patients also shifted gut bacterial diversity of these mice, an effect that was amplified during experimental colitis. VLPs isolated from UC patients specifically altered the relative abundance of several bacterial taxa previously implicated in IBD progression. Additionally, UC VLP administration heightened colitis severity in HMA mice, as indicated by shortened colon length and increased pro-inflammatory cytokine production. Importantly, this effect was dependent on intact VLPs. Our findings build on recent literature indicating that phages are dynamic regulators of bacterial communities in the gut and implicate the intestinal virome in modulating intestinal inflammation and disease.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵6 Lead contact

  • https://github.com/MauriceLab/phage_colitis

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
Back to top
PreviousNext
Posted September 11, 2021.
Download PDF

Supplementary Material

Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Transplantation of bacteriophages from ulcerative colitis patients shifts the gut bacteriome and exacerbates severity of DSS-colitis
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Transplantation of bacteriophages from ulcerative colitis patients shifts the gut bacteriome and exacerbates severity of DSS-colitis
Anshul Sinha, Yue Li, Mohammadali Khan Mirzaei, Michael Shamash, Rana Samadfam, Irah L. King, Corinne F. Maurice
bioRxiv 2021.09.10.459444; doi: https://doi.org/10.1101/2021.09.10.459444
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Transplantation of bacteriophages from ulcerative colitis patients shifts the gut bacteriome and exacerbates severity of DSS-colitis
Anshul Sinha, Yue Li, Mohammadali Khan Mirzaei, Michael Shamash, Rana Samadfam, Irah L. King, Corinne F. Maurice
bioRxiv 2021.09.10.459444; doi: https://doi.org/10.1101/2021.09.10.459444

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4842)
  • Biochemistry (10770)
  • Bioengineering (8030)
  • Bioinformatics (27243)
  • Biophysics (13956)
  • Cancer Biology (11106)
  • Cell Biology (16025)
  • Clinical Trials (138)
  • Developmental Biology (8768)
  • Ecology (13263)
  • Epidemiology (2067)
  • Evolutionary Biology (17337)
  • Genetics (11677)
  • Genomics (15901)
  • Immunology (11010)
  • Microbiology (26028)
  • Molecular Biology (10624)
  • Neuroscience (56443)
  • Paleontology (417)
  • Pathology (1729)
  • Pharmacology and Toxicology (2999)
  • Physiology (4538)
  • Plant Biology (9614)
  • Scientific Communication and Education (1612)
  • Synthetic Biology (2682)
  • Systems Biology (6967)
  • Zoology (1508)