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Role of Disulfide Bonds in Membrane Partitioning of a Viral Peptide

View ORCID ProfileSamapan Sikdar, Manidipa Banerjee, Satyavani Vemparala
doi: https://doi.org/10.1101/2021.09.21.461184
Samapan Sikdar
1The Institute of Mathematical Sciences, C.I.T. Campus, Taramani, Chennai 600113, India
2Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, 400094, India
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  • For correspondence: samapans@imsc.res.in mbanerjee@bioschool.iitd.ac.in vani@imsc.res.in
Manidipa Banerjee
3Kusuma School of Biological Sciences, Indian Institute of Technology-Delhi, Hauz Khas, New Delhi - 110016, India
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  • For correspondence: samapans@imsc.res.in mbanerjee@bioschool.iitd.ac.in vani@imsc.res.in
Satyavani Vemparala
1The Institute of Mathematical Sciences, C.I.T. Campus, Taramani, Chennai 600113, India
2Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, 400094, India
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  • For correspondence: samapans@imsc.res.in mbanerjee@bioschool.iitd.ac.in vani@imsc.res.in
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Abstract

The importance of disulfide bond in mediating viral peptide entry into host cells is well known. In the present work, we elucidate the role of disulfide (SS) bond in partitioning mechanism of membrane active Hepatitis A Virus-2B (HAV-2B) peptide, which harbours three cysteine residues promoting formation of multiple SS-bonded states. The inclusion of SS-bond not only results in a compact conformation but also induces distorted α-helical hairpin geometry in comparison to SS-free state, resulting in reduced hydrophobic exposure. Owing to this, the partitioning of HAV-2B peptide is completely or partly abolished. In a way, the disulfide bond regulates the partitioning of HAV-2B peptide, such that the membrane remodelling effects of this viral peptide are significantly reduced. The current findings may have potential implications in drug designing, targeting the HAV-2B protein by promoting disulfide bond formation within its membrane active region.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 21, 2021.
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Role of Disulfide Bonds in Membrane Partitioning of a Viral Peptide
Samapan Sikdar, Manidipa Banerjee, Satyavani Vemparala
bioRxiv 2021.09.21.461184; doi: https://doi.org/10.1101/2021.09.21.461184
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Role of Disulfide Bonds in Membrane Partitioning of a Viral Peptide
Samapan Sikdar, Manidipa Banerjee, Satyavani Vemparala
bioRxiv 2021.09.21.461184; doi: https://doi.org/10.1101/2021.09.21.461184

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