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Global fitting for high-accuracy multi-channel single-molecule localization

Yiming Li, Wei Shi, Sheng Liu, Ulf Matti, Decheng Wu, Jonas Ries
doi: https://doi.org/10.1101/2021.09.22.461230
Yiming Li
1Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China
2European Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg 69117, Germany
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  • For correspondence: liym2019@sustech.edu.cn jonas.ries@embl.de
Wei Shi
1Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China
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Sheng Liu
2European Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg 69117, Germany
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Ulf Matti
2European Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg 69117, Germany
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Decheng Wu
1Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China
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Jonas Ries
2European Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg 69117, Germany
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  • For correspondence: liym2019@sustech.edu.cn jonas.ries@embl.de
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Abstract

Multi-channel detection in single-molecule localization microscopy (SMLM) greatly increases information content for various biological applications. Here, we present globLoc, a graphics processing unit (GPU) based global fitting algorithm with flexible PSF modeling and parameter sharing, to extract maximum information from multi-channel single molecule data. We show, both in simulations and experiments, that global fitting can substantially improve the 3D localization precision for biplane and 4Pi SMLM and color assignment for ratiometric multicolor imaging.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://www.embl.de/download/ries/globLoc/

  • https://github.com/jries/SMAP/tree/develop/fit3Dcspline/GlobLoc

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 22, 2021.
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Global fitting for high-accuracy multi-channel single-molecule localization
Yiming Li, Wei Shi, Sheng Liu, Ulf Matti, Decheng Wu, Jonas Ries
bioRxiv 2021.09.22.461230; doi: https://doi.org/10.1101/2021.09.22.461230
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Global fitting for high-accuracy multi-channel single-molecule localization
Yiming Li, Wei Shi, Sheng Liu, Ulf Matti, Decheng Wu, Jonas Ries
bioRxiv 2021.09.22.461230; doi: https://doi.org/10.1101/2021.09.22.461230

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