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Ubiquitin Proteasome Pathway annotation in Diaphorina citri can reveal potential targets for RNAi based pest management

View ORCID ProfileWill Tank, View ORCID ProfileTeresa Shippy, Amanda Thate, View ORCID ProfileCrissy Massimino, View ORCID ProfilePrashant S. Hosmani, View ORCID ProfileMirella Flores-Gonzalez, View ORCID ProfileLukas A. Mueller, View ORCID ProfileWayne B. Hunter, View ORCID ProfileSusan J. Brown, View ORCID ProfileTom D’Elia, View ORCID ProfileSurya Saha
doi: https://doi.org/10.1101/2021.10.11.464014
Will Tank
1Division of Biology, Kansas State University, Manhattan, KS 66506, USA
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Teresa Shippy
1Division of Biology, Kansas State University, Manhattan, KS 66506, USA
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Amanda Thate
1Division of Biology, Kansas State University, Manhattan, KS 66506, USA
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Crissy Massimino
2Indian River State College, Fort Pierce, FL 34981, USA
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Prashant S. Hosmani
3Boyce Thompson Institute, Ithaca, NY 14853, USA
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Mirella Flores-Gonzalez
3Boyce Thompson Institute, Ithaca, NY 14853, USA
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Lukas A. Mueller
3Boyce Thompson Institute, Ithaca, NY 14853, USA
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Wayne B. Hunter
4USDA-ARS, US Horticultural Research Laboratory, Fort Pierce, FL 34945, USA
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Susan J. Brown
1Division of Biology, Kansas State University, Manhattan, KS 66506, USA
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Tom D’Elia
2Indian River State College, Fort Pierce, FL 34981, USA
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Surya Saha
3Boyce Thompson Institute, Ithaca, NY 14853, USA
6Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ 85721, USA
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  • For correspondence: ss2489@cornell.edu
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Abstract

Ubiquitination is an ATP-dependent process that targets proteins for degradation by the proteasome. In this study, we annotated 15 genes from the ubiquitin-proteasome pathway in the Asian citrus psyllid, Diaphorina citri. This psyllid vector has come to prominence in the last decade due to its role in the transmission of the devastating bacterial pathogen, Candidatus Liberibacter asiaticus (CLas). Infection of citrus crops by this pathogen causes Huanglongbing (HLB or citrus greening disease) and results in the eventual death of citrus trees. The identification and correct annotation of these genes in D. citri will be useful for functional genomic studies that aid in the development of RNAi-based management strategies aimed at reducing the spread of HLB. Investigating the effects of CLas infection on the expression of ubiquitin-proteasome pathway genes may provide new information regarding the role that these genes play in the acquisition and transmission of CLas by D. citri.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://citrusgreening.org/annotation/index

  • List of Abbreviations

    D. melanogaster
    Drosophila melanogaster;
    T. castaneum
    Tribolium castaneum;
    D. citri
    Diaphorina citri:
    CLas
    Candidatus Liberibacter asiaticus;
    NCBI
    National Center for Biotechnology Information,
    CGEN
    Citrus Greening Expression Network;
    RNAi
    RNA interference;
    RNA-seq
    RNA sequencing;
    Iso-seq
    Isoform sequencing;
    MCOT
    Maker, Cufflinks, Oasis, Trinity.
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    Ubiquitin Proteasome Pathway annotation in Diaphorina citri can reveal potential targets for RNAi based pest management
    Will Tank, Teresa Shippy, Amanda Thate, Crissy Massimino, Prashant S. Hosmani, Mirella Flores-Gonzalez, Lukas A. Mueller, Wayne B. Hunter, Susan J. Brown, Tom D’Elia, Surya Saha
    bioRxiv 2021.10.11.464014; doi: https://doi.org/10.1101/2021.10.11.464014
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    Ubiquitin Proteasome Pathway annotation in Diaphorina citri can reveal potential targets for RNAi based pest management
    Will Tank, Teresa Shippy, Amanda Thate, Crissy Massimino, Prashant S. Hosmani, Mirella Flores-Gonzalez, Lukas A. Mueller, Wayne B. Hunter, Susan J. Brown, Tom D’Elia, Surya Saha
    bioRxiv 2021.10.11.464014; doi: https://doi.org/10.1101/2021.10.11.464014

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