Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Long-term evolution of antibiotic persistence in P. aeruginosa lung infections

View ORCID ProfileMelanie Ghoul, Sandra B. Andersen, View ORCID ProfileHelle Krogh Johansen, Lars Jelsbak, Søren Molin, Gabriel Perron, Ashleigh S. Griffin
doi: https://doi.org/10.1101/2021.10.14.464434
Melanie Ghoul
1Department of Zoology, University of Oxford, OX1 3PS, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Melanie Ghoul
  • For correspondence: melanie.ghoul@zoo.ox.ac.uk
Sandra B. Andersen
2Center for Evolutionary Hologenomics, Globe Institute, University of Copenhagen, Øster Farimagsgade 5, 1353 Cph K, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Helle Krogh Johansen
3Department of Clinical Microbiology, Afsnit 9301, Rigshospitalet, Copenhagen Ø, Denmark
4Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Helle Krogh Johansen
Lars Jelsbak
5Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Søren Molin
4Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gabriel Perron
6Department of Biology, Bard College, Annandale-On-Hudson, NY, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ashleigh S. Griffin
1Department of Zoology, University of Oxford, OX1 3PS, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Pathogenic bacteria respond to antibiotic pressure with the evolution of resistance but survival can also depend on their ability to tolerate antibiotic treatment, known as persistence. While a variety of resistance mechanisms and underlying genetics are well characterised in vitro and in vivo, the evolution of persistence, and how it interacts with resistance in situ is less well understood. We assayed for persistence and resistance with three clinically relevant antibiotics: meropenem, ciprofloxacin and tobramycin, in isolates of Pseudomonas aeruginosa from chronic cystic fibrosis lung infections spanning up to forty years of evolution. We find evidence that persistence is under positive selection in the lung and that it can particularly act as an evolutionary stepping stone to resistance. However, this pattern is not universal and depends on the bacterial clone type and antibiotic used, indicating an important role for antibiotic mode of action.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted October 17, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Long-term evolution of antibiotic persistence in P. aeruginosa lung infections
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Long-term evolution of antibiotic persistence in P. aeruginosa lung infections
Melanie Ghoul, Sandra B. Andersen, Helle Krogh Johansen, Lars Jelsbak, Søren Molin, Gabriel Perron, Ashleigh S. Griffin
bioRxiv 2021.10.14.464434; doi: https://doi.org/10.1101/2021.10.14.464434
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Long-term evolution of antibiotic persistence in P. aeruginosa lung infections
Melanie Ghoul, Sandra B. Andersen, Helle Krogh Johansen, Lars Jelsbak, Søren Molin, Gabriel Perron, Ashleigh S. Griffin
bioRxiv 2021.10.14.464434; doi: https://doi.org/10.1101/2021.10.14.464434

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Evolutionary Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4091)
  • Biochemistry (8776)
  • Bioengineering (6488)
  • Bioinformatics (23359)
  • Biophysics (11758)
  • Cancer Biology (9156)
  • Cell Biology (13257)
  • Clinical Trials (138)
  • Developmental Biology (7418)
  • Ecology (11376)
  • Epidemiology (2066)
  • Evolutionary Biology (15096)
  • Genetics (10404)
  • Genomics (14014)
  • Immunology (9130)
  • Microbiology (22072)
  • Molecular Biology (8783)
  • Neuroscience (47402)
  • Paleontology (350)
  • Pathology (1421)
  • Pharmacology and Toxicology (2482)
  • Physiology (3706)
  • Plant Biology (8055)
  • Scientific Communication and Education (1433)
  • Synthetic Biology (2211)
  • Systems Biology (6017)
  • Zoology (1251)