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Cofilin regulates actin network homeostasis and microvilli length in mouse oocytes

Anne Bourdais, View ORCID ProfileBenoit Dehapiot, View ORCID ProfileGuillaume Halet
doi: https://doi.org/10.1101/2021.10.15.464498
Anne Bourdais
Univ Rennes, CNRS, IGDR - UMR 6290, F-35000 Rennes, France
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Benoit Dehapiot
Univ Rennes, CNRS, IGDR - UMR 6290, F-35000 Rennes, France
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  • ORCID record for Benoit Dehapiot
Guillaume Halet
Univ Rennes, CNRS, IGDR - UMR 6290, F-35000 Rennes, France
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  • ORCID record for Guillaume Halet
  • For correspondence: guillaume.halet@univ-rennes1.fr
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Abstract

How multiple actin networks coexist in a common cytoplasm, while competing for a shared pool of monomers, is still an ongoing question. This is exemplified by meiotic maturation in the mouse oocyte, which relies on the dynamic remodeling of distinct cortical and cytoplasmic F-actin networks. Here we show that the conserved actin-depolymerizing factor cofilin is activated in a switch-like manner at meiosis resumption from prophase arrest. Interfering with cofilin activation during maturation resulted in widespread microvilli elongation, while cytoplasmic F-actin was depleted, leading to defects in spindle migration and polar body extrusion. In contrast, cofilin inactivation in metaphase II-arrested oocytes resulted in a shutdown of F-actin dynamics, along with a dramatic overgrowth of the polarized actin cap. However, inhibition of the Arp2/3 complex to promote actin cap disassembly elicited ectopic microvilli outgrowth in the polarized cortex. These data establish cofilin as a key player in actin network homeostasis in oocytes, and reveal that microvilli can act as a sink for monomers upon disassembly of a competing network.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted October 15, 2021.
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Cofilin regulates actin network homeostasis and microvilli length in mouse oocytes
Anne Bourdais, Benoit Dehapiot, Guillaume Halet
bioRxiv 2021.10.15.464498; doi: https://doi.org/10.1101/2021.10.15.464498
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Cofilin regulates actin network homeostasis and microvilli length in mouse oocytes
Anne Bourdais, Benoit Dehapiot, Guillaume Halet
bioRxiv 2021.10.15.464498; doi: https://doi.org/10.1101/2021.10.15.464498

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