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Mass-selective and ice-free cryo-EM protein sample preparation via native electrospray ion-beam deposition
View ORCID ProfileTim K. Esser, View ORCID ProfileJan Böhning, Paul Fremdling, Mark T. Agasid, View ORCID ProfileAdam Costin, View ORCID ProfileKyle Fort, Albert Konijnenberg, Alan Bahm, View ORCID ProfileAlexander Makarov, View ORCID ProfileCarol V. Robinson, View ORCID ProfileJustin L. P. Benesch, Lindsay Baker, View ORCID ProfileTanmay A.M. Bharat, View ORCID ProfileJoseph Gault, View ORCID ProfileStephan Rauschenbach
doi: https://doi.org/10.1101/2021.10.18.464782
Tim K. Esser
1Department of Chemistry, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK
Jan Böhning
2Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
Paul Fremdling
1Department of Chemistry, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK
Mark T. Agasid
1Department of Chemistry, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK
Adam Costin
3Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK; Australian Centre for Microscopy and Microanalysis, University of Sydney, Sydney, NSW 2006, Australia
Kyle Fort
4Thermo Fisher Scientific, Bremen, 28199, Germany
Albert Konijnenberg
5Thermo Fisher Scientific, Zwaanstraat 31G/H, 5651 CA, Eindhoven, The Netherlands
Alan Bahm
6Thermo Fisher Scientific, 5350 NE Dawson Creek Drive, Hillsboro, OR, 97124, USA
Alexander Makarov
4Thermo Fisher Scientific, Bremen, 28199, Germany
7Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
Carol V. Robinson
1Department of Chemistry, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK
Justin L. P. Benesch
1Department of Chemistry, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK
Lindsay Baker
8Division of Structural Biology, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, UK; Kavli Institute for Nanoscience Discovery, Department of Biochemistry, University of Oxford, Oxford, OX1 3QZ, UK
Tanmay A.M. Bharat
2Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
9Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK
Joseph Gault
1Department of Chemistry, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK
10Vertex Pharmaceuticals, 86-88 Jubilee Avenue, Milton Park, Abingdon, OX14 4RW, UK
Stephan Rauschenbach
1Department of Chemistry, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK
11Max Planck Institute for Solid State Research, Heisenbergstrasse 1, Stuttgart, DE-70569, Germany
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Posted October 19, 2021.
Mass-selective and ice-free cryo-EM protein sample preparation via native electrospray ion-beam deposition
Tim K. Esser, Jan Böhning, Paul Fremdling, Mark T. Agasid, Adam Costin, Kyle Fort, Albert Konijnenberg, Alan Bahm, Alexander Makarov, Carol V. Robinson, Justin L. P. Benesch, Lindsay Baker, Tanmay A.M. Bharat, Joseph Gault, Stephan Rauschenbach
bioRxiv 2021.10.18.464782; doi: https://doi.org/10.1101/2021.10.18.464782
Mass-selective and ice-free cryo-EM protein sample preparation via native electrospray ion-beam deposition
Tim K. Esser, Jan Böhning, Paul Fremdling, Mark T. Agasid, Adam Costin, Kyle Fort, Albert Konijnenberg, Alan Bahm, Alexander Makarov, Carol V. Robinson, Justin L. P. Benesch, Lindsay Baker, Tanmay A.M. Bharat, Joseph Gault, Stephan Rauschenbach
bioRxiv 2021.10.18.464782; doi: https://doi.org/10.1101/2021.10.18.464782
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