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Opposing subclasses of Drosophila ellipsoid body neurons promote and suppress sleep

Abigail Aleman, Jaison Jiro Omoto, Prabhjit Singh, Bao-Chau Nguyen, Pratyush Kandimalla, Volker Hartenstein, View ORCID ProfileJeffrey M. Donlea
doi: https://doi.org/10.1101/2021.10.19.464469
Abigail Aleman
1Department of Neurobiology, David Geffen School of Medicine at University of California – Los Angeles, Los Angeles, CA, 90095, United States
2Molecular, Cellular & Integrative Physiology Interdepartmental Program, University of California – Los Angeles, Los Angeles, CA, 90095, United States
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Jaison Jiro Omoto
1Department of Neurobiology, David Geffen School of Medicine at University of California – Los Angeles, Los Angeles, CA, 90095, United States
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Prabhjit Singh
1Department of Neurobiology, David Geffen School of Medicine at University of California – Los Angeles, Los Angeles, CA, 90095, United States
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Bao-Chau Nguyen
3Department of Molecular, Cellular, and Developmental Biology, University of California – Los Angeles, Los Angeles, CA, 90095, United States
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Pratyush Kandimalla
3Department of Molecular, Cellular, and Developmental Biology, University of California – Los Angeles, Los Angeles, CA, 90095, United States
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Volker Hartenstein
3Department of Molecular, Cellular, and Developmental Biology, University of California – Los Angeles, Los Angeles, CA, 90095, United States
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Jeffrey M. Donlea
1Department of Neurobiology, David Geffen School of Medicine at University of California – Los Angeles, Los Angeles, CA, 90095, United States
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  • ORCID record for Jeffrey M. Donlea
  • For correspondence: jdonlea@ucla.edu
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Summary

Recent work in Drosophila has uncovered several neighboring classes of sleep-regulatory neurons within the central complex. However, the logic of connectivity and network motifs remains limited by the incomplete examination of relevant cell types. Using a recent genetic-anatomical classification of ellipsoid body ring neurons, we conducted a thermogenetic screen to assess sleep/wake behavior and discovered two opposing populations: sleep-promoting R3m and wake-promoting R3d neurons. Activation of these neurons influences sleep duration and architecture by prolonging or shortening sleep bouts, suggesting a key role in sleep maintenance. R3m and R3d neurons are GABAergic and require GABA synthesis for their effects on sleep. Finally, we use a fluorescent reporter for putative synaptic partners to embed these neurons within the known sleep-regulatory network; R3m and R3d neurons lay downstream of wake-active Helicon cells, and R3m neurons likely inhibit R3d neurons. Together, the data presented herein suggest a neural mechanism by which previously uncharacterized circuit elements operate within the sleep homeostat to stabilize sleep-wake states.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵4 Lead Contact: jdonlea{at}ucla.edu

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 19, 2021.
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Opposing subclasses of Drosophila ellipsoid body neurons promote and suppress sleep
Abigail Aleman, Jaison Jiro Omoto, Prabhjit Singh, Bao-Chau Nguyen, Pratyush Kandimalla, Volker Hartenstein, Jeffrey M. Donlea
bioRxiv 2021.10.19.464469; doi: https://doi.org/10.1101/2021.10.19.464469
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Opposing subclasses of Drosophila ellipsoid body neurons promote and suppress sleep
Abigail Aleman, Jaison Jiro Omoto, Prabhjit Singh, Bao-Chau Nguyen, Pratyush Kandimalla, Volker Hartenstein, Jeffrey M. Donlea
bioRxiv 2021.10.19.464469; doi: https://doi.org/10.1101/2021.10.19.464469

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