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Single-particle tracking of dynein identifies PP2A B55/SUR-6 as a cell cycle regulator of cortical force generation

View ORCID ProfileAlan Edwards, View ORCID ProfileJohn B. Linehan, View ORCID ProfileVincent Boudreau, View ORCID ProfilePaul S. Maddox
doi: https://doi.org/10.1101/2021.10.22.465443
Alan Edwards
1UNC-Chapel Hill
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John B. Linehan
1UNC-Chapel Hill
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Vincent Boudreau
2University of California, Berkeley, CA 94720 and Department of Biochemistry & Biophysics University of California
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Paul S. Maddox
1UNC-Chapel Hill
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  • For correspondence: pmaddox@email.unc.edu
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Abstract

Convergence and positioning of the pronuclei and mitotic spindle of many zygotes aids efficient division and is essential for early embryonic patterning. In the C. elegans zygote, interactions between microtubules and cortically anchored dynein are key to early development. However, how cortical microtubule pulling forces are controlled through the cell cycle is less well understood. We used single-molecule imaging and a windowed mean squared displacement analysis to uncover the behavior of dynein during cortical force generation, and provide a regulatory role for protein phosphatase PP2A-B55/SUR6 via NuMA-like protein LIN-5 in this process. Previous findings and our results suggest that PP2A regulates cortical microtubule pulling forces by increasing dynein binding and unbinding to the cortical force generation complex. Our data also suggests that cortical occupancy of dynein is abrogated to vary force generation. Our approach will be broadly applicable to classify the force generation behavior of single molecules in living organisms.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted November 10, 2021.
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Single-particle tracking of dynein identifies PP2A B55/SUR-6 as a cell cycle regulator of cortical force generation
Alan Edwards, John B. Linehan, Vincent Boudreau, Paul S. Maddox
bioRxiv 2021.10.22.465443; doi: https://doi.org/10.1101/2021.10.22.465443
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Single-particle tracking of dynein identifies PP2A B55/SUR-6 as a cell cycle regulator of cortical force generation
Alan Edwards, John B. Linehan, Vincent Boudreau, Paul S. Maddox
bioRxiv 2021.10.22.465443; doi: https://doi.org/10.1101/2021.10.22.465443

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