Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing

View ORCID ProfileMantas Sereika, View ORCID ProfileRasmus Hansen Kirkegaard, View ORCID ProfileSøren Michael Karst, View ORCID ProfileThomas Yssing Michaelsen, View ORCID ProfileEmil Aarre Sørensen, View ORCID ProfileRasmus Dam Wollenberg, View ORCID ProfileMads Albertsen
doi: https://doi.org/10.1101/2021.10.27.466057
Mantas Sereika
aCenter for microbial communities, Aalborg University, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mantas Sereika
Rasmus Hansen Kirkegaard
aCenter for microbial communities, Aalborg University, Denmark
bJoint Microbiome Facility, University of Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Rasmus Hansen Kirkegaard
Søren Michael Karst
aCenter for microbial communities, Aalborg University, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Søren Michael Karst
Thomas Yssing Michaelsen
aCenter for microbial communities, Aalborg University, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Thomas Yssing Michaelsen
Emil Aarre Sørensen
aCenter for microbial communities, Aalborg University, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Emil Aarre Sørensen
Rasmus Dam Wollenberg
cDNASense ApS, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Rasmus Dam Wollenberg
Mads Albertsen
aCenter for microbial communities, Aalborg University, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mads Albertsen
  • For correspondence: ma@bio.aau.dk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-perfect genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-perfect microbial genomes from isolates or metagenomes without shortread or reference polishing.

Competing Interest Statement

EAS, SMK, MA, RHK, and RWO are also employed at DNASense ApS. The remaining authors declare no conflict of interest.

Footnotes

  • The manuscript has been completely revised and restructured to include Nanopore R10.4 data.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted November 10, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing
Mantas Sereika, Rasmus Hansen Kirkegaard, Søren Michael Karst, Thomas Yssing Michaelsen, Emil Aarre Sørensen, Rasmus Dam Wollenberg, Mads Albertsen
bioRxiv 2021.10.27.466057; doi: https://doi.org/10.1101/2021.10.27.466057
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing
Mantas Sereika, Rasmus Hansen Kirkegaard, Søren Michael Karst, Thomas Yssing Michaelsen, Emil Aarre Sørensen, Rasmus Dam Wollenberg, Mads Albertsen
bioRxiv 2021.10.27.466057; doi: https://doi.org/10.1101/2021.10.27.466057

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4113)
  • Biochemistry (8815)
  • Bioengineering (6519)
  • Bioinformatics (23462)
  • Biophysics (11789)
  • Cancer Biology (9209)
  • Cell Biology (13323)
  • Clinical Trials (138)
  • Developmental Biology (7436)
  • Ecology (11409)
  • Epidemiology (2066)
  • Evolutionary Biology (15151)
  • Genetics (10436)
  • Genomics (14043)
  • Immunology (9171)
  • Microbiology (22154)
  • Molecular Biology (8812)
  • Neuroscience (47569)
  • Paleontology (350)
  • Pathology (1428)
  • Pharmacology and Toxicology (2491)
  • Physiology (3730)
  • Plant Biology (8080)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2221)
  • Systems Biology (6037)
  • Zoology (1253)