Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A biosafety level 2 surrogate for studying SARS-CoV-2 survival in food processing environmental biofilms

Austin B. Featherstone, Sapna Chitlapilly Dass
doi: https://doi.org/10.1101/2021.10.29.466519
Austin B. Featherstone
Department of Animal Science, Texas A&M University, College Station, Texas 77845
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sapna Chitlapilly Dass
Department of Animal Science, Texas A&M University, College Station, Texas 77845
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: sapna.dass@agnet.tamu.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Meat processing plants have been at the center of the SARS-CoV-2 pandemic. There are several factors that contribute to the persistence of SARS-CoV-2 in meat processing plants and one of the factors is the formation of a multi-species biofilm with virus. Biofilm can act as a reservoir in protecting, harboring, and dispersing SARS-CoV-2 from biofilm to the meat processing facility environment. We used Murine Hepatitis Virus (MHV) as a surrogate for SARS-CoV-2 virus and meat processing facility drain samples to develop mixed-species biofilms on commonly found materials in processing facilities (Stainless-Steel (SS), PVC and tiles). The results showed that MHV was able to integrate into the environmental biofilm and survived for a period of 5 days at 7°C. There was no significate difference between the viral-environmental biofilm biovolumes developed on different materials SS, PVC, and tiles. There was a 2-fold increase in the virus-environmental biofilm biovolume when compared to environmental biofilm by itself. These results indicate a complex virus-environmental biofilm interaction which is providing enhanced protection for the survival of viral particles with the environmental biofilm community.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted November 01, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A biosafety level 2 surrogate for studying SARS-CoV-2 survival in food processing environmental biofilms
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A biosafety level 2 surrogate for studying SARS-CoV-2 survival in food processing environmental biofilms
Austin B. Featherstone, Sapna Chitlapilly Dass
bioRxiv 2021.10.29.466519; doi: https://doi.org/10.1101/2021.10.29.466519
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
A biosafety level 2 surrogate for studying SARS-CoV-2 survival in food processing environmental biofilms
Austin B. Featherstone, Sapna Chitlapilly Dass
bioRxiv 2021.10.29.466519; doi: https://doi.org/10.1101/2021.10.29.466519

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4395)
  • Biochemistry (9619)
  • Bioengineering (7110)
  • Bioinformatics (24915)
  • Biophysics (12642)
  • Cancer Biology (9979)
  • Cell Biology (14386)
  • Clinical Trials (138)
  • Developmental Biology (7968)
  • Ecology (12133)
  • Epidemiology (2067)
  • Evolutionary Biology (16008)
  • Genetics (10937)
  • Genomics (14764)
  • Immunology (9889)
  • Microbiology (23718)
  • Molecular Biology (9493)
  • Neuroscience (50964)
  • Paleontology (370)
  • Pathology (1544)
  • Pharmacology and Toxicology (2688)
  • Physiology (4031)
  • Plant Biology (8677)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2403)
  • Systems Biology (6446)
  • Zoology (1346)